Universität zu Köln

Schmidt, Luisa ORCID: 0000-0002-9805-4941, Saynisch, Michael ORCID: 0000-0001-8785-4081, Hoegsbjerg, Christian, Schmidt, Andreas ORCID: 0000-0001-9369-3747, Mackey, Abigail, Lackmann, Jan-Wilm ORCID: 0000-0001-8182-8034, Müller, Stefan ORCID: 0000-0002-9813-4685, Koch, Manuel ORCID: 0000-0002-2962-7814, Brachvogel, Bent ORCID: 0000-0002-3923-0554, Kjaer, Michael, Antczak, Philipp ORCID: 0000-0001-9600-7757 and Krüger, Marcus ORCID: 0000-0002-5846-6941 (2024). Spatial proteomics of skeletal muscle using thin cryosections reveals metabolic adaptation at the muscle-tendon transition zone. Cell Reports, 43 (7). pp. 1-22. Elsevier. ISSN 2211-1247

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Abstract

[Artikel-Nr.: 114374] Morphological studies of skeletal muscle tissue provide insights into the architecture of muscle fibers, the surrounding cells, and the extracellular matrix (ECM). However, a spatial proteomics analysis of the skeletal muscle including the muscle-tendon transition zone is lacking. Here, we prepare cryotome muscle sections of the mouse soleus muscle and measure each slice using short liquid chromatography-mass spectrometry (LC-MS) gradients. We generate 3,000 high-resolution protein profiles that serve as the basis for a network analysis to reveal the complex architecture of the muscle-tendon junction. Among the protein profiles that increase from muscle to tendon, we find proteins related to neuronal activity, fatty acid biosynthesis, and the renin-angiotensin system (RAS). Blocking the RAS in cultured mouse tenocytes using losartan reduces the ECM synthesis. Overall, our analysis of thin cryotome sections provides a spatial proteome of skeletal muscle and reveals that the RAS acts as an additional regulator of the matrix within muscle-tendon junctions.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Schmidt, Luisa UNSPECIFIED https://orcid.org/0000-0002-9805-4941 UNSPECIFIED Saynisch, Michael UNSPECIFIED https://orcid.org/0000-0001-8785-4081 UNSPECIFIED Hoegsbjerg, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Andreas UNSPECIFIED https://orcid.org/0000-0001-9369-3747 UNSPECIFIED Mackey, Abigail UNSPECIFIED UNSPECIFIED UNSPECIFIED Lackmann, Jan-Wilm UNSPECIFIED https://orcid.org/0000-0001-8182-8034 UNSPECIFIED Müller, Stefan UNSPECIFIED https://orcid.org/0000-0002-9813-4685 UNSPECIFIED Koch, Manuel UNSPECIFIED https://orcid.org/0000-0002-2962-7814 UNSPECIFIED Brachvogel, Bent UNSPECIFIED https://orcid.org/0000-0002-3923-0554 UNSPECIFIED Kjaer, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Antczak, Philipp UNSPECIFIED https://orcid.org/0000-0001-9600-7757 UNSPECIFIED Krüger, Marcus UNSPECIFIED https://orcid.org/0000-0002-5846-6941 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-813775
Identification Number:	10.1016/j.celrep.2024.114374
Journal or Publication Title:	Cell Reports
Volume:	43
Number:	7
Page Range:	pp. 1-22
Number of Pages:	22
Date:	23 July 2024
Publisher:	Elsevier
ISSN:	2211-1247
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Biochemie > Zentrum für Biochemie Faculty of Medicine > Kinder- und Jugendmedizin > Klinik und Poliklinik für Kinder- und Jugendmedizin Zentrum für Molekulare Medizin
Subjects:	Life sciences Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language spatial proteomics ; skeletal muscle ; myotendinous junction ; distance-based network English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81377