Universität zu Köln

Abu Obaid, Asmaa ORCID: 0009-0006-1291-8371, Ivandic, Ivan and Korsching, Sigrun I. ORCID: 0000-0002-5450-172X (2024). Deciphering the function of the fifth class of Gα proteins: regulation of ionic homeostasis as unifying hypothesis. Cellular and Molecular Life Sciences, 81 (1). pp. 1-15. Springer Nature. ISSN 1420-682X

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Abstract

[Artikel-Nr. 213] Trimeric G proteins transduce signals from a superfamily of receptors and each G protein controls a wide range of cellular and systemic functions. Their highly conserved alpha subunits fall in five classes, four of which have been well investigated (Gs, Gi, G12, Gq). In contrast, the function of the fifth class, Gv is completely unknown, despite its broad occurrence and evolutionary ancient origin (older than metazoans). Here we show a dynamic presence of Gv mRNA in several organs during early development of zebrafish, including the hatching gland, the pronephros and several cartilage anlagen, employing in situ hybridisation. Next, we generated a Gv frameshift mutation in zebrafish and observed distinct phenotypes such as reduced oviposition, premature hatching and craniofacial abnormalities in bone and cartilage of larval zebrafish. These phenotypes could suggest a disturbance in ionic homeostasis as a common denominator. Indeed, we find reduced levels of calcium, magnesium and potassium in the larvae and changes in expression levels of the sodium potassium pump atp1a1a.5 and the sodium/calcium exchanger ncx1b in larvae and in the adult kidney, a major osmoregulatory organ. Additionally, expression of sodium chloride cotransporter slc12a3 and the anion exchanger slc26a4 is altered in complementary ways in adult kidney. It appears that Gv may modulate ionic homeostasis in zebrafish during development and in adults. Our results constitute the first insight into the function of the fifth class of G alpha proteins.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Abu Obaid, Asmaa UNSPECIFIED https://orcid.org/0009-0006-1291-8371 UNSPECIFIED Ivandic, Ivan UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun I. UNSPECIFIED https://orcid.org/0000-0002-5450-172X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-814693
Identification Number:	10.1007/s00018-024-05228-w
Journal or Publication Title:	Cellular and Molecular Life Sciences
Volume:	81
Number:	1
Page Range:	pp. 1-15
Number of Pages:	15
Date:	10 December 2024
Publisher:	Springer Nature
ISSN:	1420-682X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	Life sciences
Uncontrolled Keywords:	Keywords Language G protein-coupled receptor ; Gnav ; Osmoregulation ; Signal transduction ; Oogenesis English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81469