Universität zu Köln

Wang, Xiaosha ORCID: 0009-0003-8044-3951, Frühn, Layla ORCID: 0009-0005-7798-6175, Li, Panpan ORCID: 0000-0001-5777-0899, Shi, Xin ORCID: 0000-0002-6649-4045, Wang, Nini ORCID: 0000-0002-3698-5986, Feng, Yuan, Prinz, Julia, Liu, Hanhan ORCID: 0000-0003-0204-1087 and Prokosch, Verena ORCID: 0000-0002-5318-0637 (2024). Comparative proteomic analysis of regenerative mechanisms in mouse retina to identify markers for neuro-regeneration in glaucoma. Scientific Reports, 14 (1). pp. 1-17. Springer Nature. ISSN 2045-2322

PDF s41598-024-72378-z.pdf Bereitstellung unter der CC-Lizenz: Creative Commons Attribution. Download (4MB)

Abstract

[Artikel-Nr.: 23118] The retina is part of the central nervous system (CNS). Neurons in the CNS and retinal ganglion cells lack the ability to regenerate axons spontaneously after injury. The intrinsic axonal growth regulators, their interaction and roles that enable or inhibit axon growth are still largely unknown. This study endeavored to characterize the molecular characteristics under neurodegenerative and regenerative conditions. Data-Independent Acquisition Mass Spectrometry was used to map the comprehensive proteome of the regenerative retina from 14-day-old mice (Reg-P14) and adult mice after lens injury (Reg-LI) both showing regrowing axons in vitro, untreated adult mice, and retina from adult mice subjected to two weeks of elevated intraocular pressure showing degeneration. A total of 5750 proteins were identified (false discovery rate < 1%). Proteins identified in both Reg-P14 and Reg-LI groups were correlated to thyroid hormone, Notch, Wnt, and VEGF signaling pathways. Common interactors comprising E1A binding protein P300 (EP300), CREB binding protein (CBP), calcium/calmodulin dependent protein kinase II alpha (CaMKIIα) and sirtuin 1 (SIRT1) were found in both Reg-P14 and Reg-LI retinas. Proteins identified in both regenerating and degenerative groups were correlated to thyroid hormone, Notch, mRNA surveillance and measles signaling pathways, along with PD-L1 expression and the PD-1 checkpoint pathway. Common interactors across regenerative and degenerative retinas comprising NF-kappa-B p65 subunit (RELA), RNA-binding protein with serine-rich domain 1 (RNPS1), EP300 and SIN3 transcription regulator family member A (SIN3A). The findings from our study provide the first mapping of regenerative mechanisms across postnatal, mature and degenerative mouse retinas, revealing potential biomarkers that could facilitate neuro-regeneration in glaucoma.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Xiaosha UNSPECIFIED https://orcid.org/0009-0003-8044-3951 UNSPECIFIED Frühn, Layla UNSPECIFIED https://orcid.org/0009-0005-7798-6175 UNSPECIFIED Li, Panpan UNSPECIFIED https://orcid.org/0000-0001-5777-0899 UNSPECIFIED Shi, Xin UNSPECIFIED https://orcid.org/0000-0002-6649-4045 UNSPECIFIED Wang, Nini UNSPECIFIED https://orcid.org/0000-0002-3698-5986 UNSPECIFIED Feng, Yuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Prinz, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Hanhan UNSPECIFIED https://orcid.org/0000-0003-0204-1087 UNSPECIFIED Prokosch, Verena UNSPECIFIED https://orcid.org/0000-0002-5318-0637 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-815869
Identification Number:	10.1038/s41598-024-72378-z
Journal or Publication Title:	Scientific Reports
Volume:	14
Number:	1
Page Range:	pp. 1-17
Date:	4 October 2024
Publisher:	Springer Nature
ISSN:	2045-2322
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Augenheilkunde > Klinik und Poliklinik für Allgemeine Augenheilkunde
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language RGC regeneration ; Glaucoma ; RGC degeneration ; Mass spectrometry ; Proteomic landscape English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81586