Universität zu Köln

Pagliuso, Alessandro, Allemand, Eric, Robertin, Stevens ORCID: 0000-0001-6816-5352, Dupuy, Bruno, Bertrand, Quentin, Becavin, Christophe ORCID: 0000-0003-1555-3153, Koutero, Mikael, Najburg, Valerie, Nahori, Marie-Anne, Tangy, Frederic, Stavru, Fabrizia, Bessonov, Sergey, Dessen, Andrea ORCID: 0000-0001-6487-4020, Muchardt, Christian, Lebreton, Alice ORCID: 0000-0002-6643-902X, Komarova, Anastassia, V and Cossart, Pascale (2019). An RNA-Binding Protein Secreted by a Bacterial Pathogen Modulates RIG-I Signaling. Cell Host Microbe, 26 (6). S. 823 - 847. CAMBRIDGE: CELL PRESS. ISSN 1934-6069

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Abstract

RNA-binding proteins (RBPs) perform key cellular activities by controlling the function of bound RNAs. The widely held assumption that RBPs are strictly intracellular has been challenged by the discovery of secreted RBPs. However, extracellular RBPs have been described in eukaryotes, while secreted bacterial RBPs have not been reported. Here, we show that the bacterial pathogen Listeria monocytogenes secretes a small RBP that we named Zea. We show that Zea binds a subset of L. monocytogenes RNAs, causing their accumulation in the extracellular medium. Furthermore, during L. monocytogenes infection, Zea binds RIG-I, the non-self-RNA innate immunity sensor, potentiating interferon-beta production. Mouse infection studies reveal that Zea affects L. monocytogenes virulence. Together, our results unveil that bacterial RNAs can be present extracellularly in association with RBPs, acting as social RNAs to trigger a host response during infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pagliuso, Alessandro UNSPECIFIED UNSPECIFIED UNSPECIFIED Allemand, Eric UNSPECIFIED UNSPECIFIED UNSPECIFIED Robertin, Stevens UNSPECIFIED orcid.org/0000-0001-6816-5352 UNSPECIFIED Dupuy, Bruno UNSPECIFIED UNSPECIFIED UNSPECIFIED Bertrand, Quentin UNSPECIFIED UNSPECIFIED UNSPECIFIED Becavin, Christophe UNSPECIFIED orcid.org/0000-0003-1555-3153 UNSPECIFIED Koutero, Mikael UNSPECIFIED UNSPECIFIED UNSPECIFIED Najburg, Valerie UNSPECIFIED UNSPECIFIED UNSPECIFIED Nahori, Marie-Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Tangy, Frederic UNSPECIFIED UNSPECIFIED UNSPECIFIED Stavru, Fabrizia UNSPECIFIED UNSPECIFIED UNSPECIFIED Bessonov, Sergey UNSPECIFIED UNSPECIFIED UNSPECIFIED Dessen, Andrea UNSPECIFIED orcid.org/0000-0001-6487-4020 UNSPECIFIED Muchardt, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lebreton, Alice UNSPECIFIED orcid.org/0000-0002-6643-902X UNSPECIFIED Komarova, Anastassia, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Cossart, Pascale UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-124549
DOI:	10.1016/j.chom.2019.10.004
Journal or Publication Title:	Cell Host Microbe
Volume:	26
Number:	6
Page Range:	S. 823 - 847
Date:	2019
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1934-6069
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LISTERIA-MONOCYTOGENES; READ ALIGNMENT; REVEALS; MICRORNAS; INFECTION; GENE; HFQ Multiple languages Microbiology; Parasitology; Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12454