Universität zu Köln

Funke, Timo, von Brandenstein, Melanie, Paffenholz, Pia, Koditz, Barbara, Nestler, Tim, Herden, Jan, Montesinos-Rongen, Manuel, Isachenko, Evgenia, Rahimi, Gohar, Fries, Jochen W. U., Heidenreich, Axel and Salem, Johannes (2019). Vimentin 3 Allows Differentiation between Normozoospermia and Oligoasthenoteratozoospermia. Dis. Markers, 2019. LONDON: HINDAWI LTD. ISSN 1875-8630

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Abstract

Vimentin is a structural protein predominantly located in the head of sperms. The function and localization of the previously identified truncated version, Vimentin 3 (Vim3), are still unknown. To investigate whether the expression of Vim3 can be used as a reliable marker for the differentiation of sperm quality, we analyzed ejaculates from patients with oligoasthenoteratozoospermia (OAT) syndrome and normozoospermia. We identified sperms with head, neck, and tail changes, which were less positive for Vim3 in OAT syndrome compared to normozoospermia. The expression of Vim3 was significantly downregulated in patients with OAT syndrome compared to sperms from patients with normozoospermia (**p<0.01). The ELISA analysis showed similar results as ejaculates from normozoospermic patients showed a significantly higher Vim3 concentration than patients with OAT syndrome (***p<0.001). This study demonstrates that Vim3 is more highly expressed in ejaculates from patients with normozoospermia compared to ejaculates from patients with OAT syndrome. Therefore, we postulate that Vim3 can be used to determine ejaculate quality. Furthermore, we identified the marker, Vim3, to differentiate between mature sperms with no morphological changes and sperms with head, neck, and tail changes. A lateral flow assay that allows quick analysis is currently under development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Funke, Timo UNSPECIFIED UNSPECIFIED UNSPECIFIED von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Paffenholz, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED Koditz, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Nestler, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Herden, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Montesinos-Rongen, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidenreich, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Salem, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-124621
DOI:	10.1155/2019/9803498
Journal or Publication Title:	Dis. Markers
Volume:	2019
Date:	2019
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1875-8630
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INFERTILITY Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental; Pathology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12462