Universität zu Köln

Oliva, Ricardo, Ji, Chonghui, Atienza-Grande, Genelou, Huguet-Tapia, Jose C., Perez-Quintero, Alvaro, Li, Ting ORCID: 0000-0001-8273-5593, Eom, Joon-Seob, Li, Chenhao, Nguyen, Hanna, Liu, Bo, Auguy, Florence, Sciallano, Coline, Luu, Van T., Dossa, Gerbert S., Cunnac, Sebastien, Schmidt, Sarah M., Slamet-Loedin, Inez H., Vera Cruz, Casiana, Szurek, Boris, Frommer, Wolf B., White, Frank F. and Yang, Bing ORCID: 0000-0002-2293-3384 (2019). Broad-spectrum resistance to bacterial blight in rice using genome editing. Nat. Biotechnol., 37 (11). S. 1344 - 1353. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1546-1696

Full text not available from this repository.

Abstract

Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR-Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oliva, Ricardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Ji, Chonghui UNSPECIFIED UNSPECIFIED UNSPECIFIED Atienza-Grande, Genelou UNSPECIFIED UNSPECIFIED UNSPECIFIED Huguet-Tapia, Jose C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Perez-Quintero, Alvaro UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Ting UNSPECIFIED orcid.org/0000-0001-8273-5593 UNSPECIFIED Eom, Joon-Seob UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Chenhao UNSPECIFIED UNSPECIFIED UNSPECIFIED Nguyen, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Bo UNSPECIFIED UNSPECIFIED UNSPECIFIED Auguy, Florence UNSPECIFIED UNSPECIFIED UNSPECIFIED Sciallano, Coline UNSPECIFIED UNSPECIFIED UNSPECIFIED Luu, Van T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dossa, Gerbert S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cunnac, Sebastien UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Sarah M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Slamet-Loedin, Inez H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vera Cruz, Casiana UNSPECIFIED UNSPECIFIED UNSPECIFIED Szurek, Boris UNSPECIFIED UNSPECIFIED UNSPECIFIED Frommer, Wolf B. UNSPECIFIED UNSPECIFIED UNSPECIFIED White, Frank F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Yang, Bing UNSPECIFIED orcid.org/0000-0002-2293-3384 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-129454
DOI:	10.1038/s41587-019-0267-z
Journal or Publication Title:	Nat. Biotechnol.
Volume:	37
Number:	11
Page Range:	S. 1344 - 1353
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1546-1696
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AGROBACTERIUM-MEDIATED TRANSFORMATION; DISEASE-SUSCEPTIBILITY GENE; XANTHOMONAS-ORYZAE; TAL EFFECTOR; III EFFECTORS; CRISPR/CAS9; ARTEMIS; FAMILY Multiple languages Biotechnology & Applied Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12945