Universität zu Köln

Caisova, Lenka and Jobe, Timothy O. (2019). Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes. Plant Methods, 15. LONDON: BMC. ISSN 1746-4811

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Abstract

BackgroundGreen plants comprise two lineages: (1) the streptophytes that colonised land and (2) the chlorophytes that have adaptations to land but remained mostly aquatic. To better understand what made streptophytes so successful, we are currently establishing the chlorophyte alga Draparnaldia sp. (Chaetophorales, Chlorophyceae) as a model for comparative analyses between these two lineages. However, establishing Draparnaldia as a valuable model requires that it can be transformed. Thus, our goal is to develop a transformation protocol for this alga.ResultsWe have established the first transformation protocol for Draparnaldia. This protocol is based on protoplast transformation by electroporation. It includes instructions on protoplast isolation, regeneration and transient transfection. It also provides a list of the effective selective agents for future Draparnaldia transformations.ConclusionsOur protocol opens a way for Draparnaldia functional genomics analyses. Moreover, it also provides an important base for establishment of stable transformation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Caisova, Lenka UNSPECIFIED UNSPECIFIED UNSPECIFIED Jobe, Timothy O. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-135197
DOI:	10.1186/s13007-019-0460-6
Journal or Publication Title:	Plant Methods
Volume:	15
Date:	2019
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1746-4811
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STABLE TRANSFORMATION; CULTURE; ELECTROPORATION; CHAETOPHORALES; MUTAGENESIS; EVOLUTION; ORIGIN; ALGA; ULVA Multiple languages Biochemical Research Methods; Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13519