Ashykhmina, Natallia, Lorenz, Melanie, Frerigmann, Henning ORCID: 0000-0002-7067-2721, Koprivova, Anna ORCID: 0000-0001-8168-4536, Hofsetz, Eduard ORCID: 0000-0001-5830-4566, Stuehrwohldt, Nils, Fluegge, Ulf-Ingo, Haferkamp, Ilka, Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Gigolashvili, Tamara ORCID: 0000-0002-0416-4796 (2019). PAPST2 Plays Critical Roles in Removing the Stress Signaling Molecule 3 '-Phosphoadenosine 5 '-Phosphate from the Cytosol and Its Subsequent Degradation in Plastids and Mitochondria. Plant Cell, 31 (1). S. 231 - 250. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

The compartmentalization of PAPS (the sulfate donor 3'-phosphoadenosine 5'-phosphosulfate) synthesis (mainly in plastids), PAPS consumption (in the cytosol), and PAP (the stress signaling molecule 3'-phosphoadenosine 5'-phosphate) degradation (in plastids and mitochondria) requires organellar transport systems for both PAPS and PAP. The plastidial transporter PAPST1 (PAPS TRANSPORTER1) delivers newly synthesized PAPS from the stroma to the cytosol. We investigated the activity of PAPST2, the closest homolog of PAPST1, which unlike PAPST1 is targeted to both the plastids and mitochondria. Biochemical characterization in Arabidopsis thaliana revealed that PAPST2 mediates the antiport of PAP, PAPS, ATP, and ADP. Strongly increased cellular PAP levels negatively affect plant growth, as observed in the fry1 papst2 mutant, which lacks the PAP-catabolizing enzyme SALT TOLERANCE 1 and PAPST2. PAP levels were specifically elevated in the cytosol of papst2 and fiery1 papst2, but not in papst1 or fry1 papst1. PAPST1 failed to complement the papst2 mutant phenotype in mitochondria, because it likely removes PAPS from the cell, as demonstrated by the increased expression of phytosulfokine genes. Overexpression of SAL1 in mitochondria rescued the phenotype of fry1 but not fry1 papst2. Therefore, PAPST2 represents an important organellar importer of PAP, providing a piece of the puzzle in our understanding of the organelle-to-nucleus PAP retrograde signaling pathway.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ashykhmina, NatalliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lorenz, MelanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frerigmann, HenningUNSPECIFIEDorcid.org/0000-0002-7067-2721UNSPECIFIED
Koprivova, AnnaUNSPECIFIEDorcid.org/0000-0001-8168-4536UNSPECIFIED
Hofsetz, EduardUNSPECIFIEDorcid.org/0000-0001-5830-4566UNSPECIFIED
Stuehrwohldt, NilsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fluegge, Ulf-IngoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haferkamp, IlkaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kopriva, StanislavUNSPECIFIEDorcid.org/0000-0002-7416-6551UNSPECIFIED
Gigolashvili, TamaraUNSPECIFIEDorcid.org/0000-0002-0416-4796UNSPECIFIED
URN: urn:nbn:de:hbz:38-139602
DOI: 10.1105/tpc.18.00512
Journal or Publication Title: Plant Cell
Volume: 31
Number: 1
Page Range: S. 231 - 250
Date: 2019
Publisher: AMER SOC PLANT BIOLOGISTS
Place of Publication: ROCKVILLE
ISSN: 1532-298X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SUBCELLULAR-LOCALIZATION; 3(2),5-BISPHOSPHATE NUCLEOTIDASE; PHYTOSULFOKINE-ALPHA; ADP/ATP CARRIER; GENE-EXPRESSION; SALT TOLERANCE; ARABIDOPSIS; ACID; PROTEINS; SULFURMultiple languages
Biochemistry & Molecular Biology; Plant Sciences; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/13960

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