Universität zu Köln

Lechner, Axel, Schloesser, Hans A., Thelen, Martin ORCID: 0000-0002-2785-9726, Wennhold, Kerstin, Rothschild, Sacha, I, Gilles, Ramona, Quaas, Alexander, Siefer, Oliver G., Huebbers, Christian U., Cukuroglu, Engin, Goeke, Jonathan, Hillmer, Axel, Gathof, Birgit, Meyer, Moritz F., Klussmann, Jens P., Shimabukuro-Vornhagen, Alexander, Theurich, Sebastian ORCID: 0000-0001-5706-8258, Beutner, Dirk and von Bergwelt-Baildon, Michael (2019). Tumor-associated B cells and humoral immune response in head and neck squamous cell carcinoma. OncoImmunology, 8 (3). PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 2162-402X

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Abstract

B lymphocytes are important players in immune responses to cancer. However, their composition and function in head and neck squamous cell carcinoma (HNSCC) has not been well described. Here, we analyzed B cell subsets in HNSCC (n = 38), non-cancerous mucosa (n = 14) and peripheral blood from HNSCC patients (n = 38) and healthy controls (n = 20) by flow cytometry. Intratumoral B cells contained high percentages of activated (CD86(+)), antigen-presenting (CD86(+)/CD21(-)) and memory B cells (IgD(-)/CD27(+)). T follicular helper cells (CD4(+)/CXCR5(+)/CD45RA(-)/CCR7(-)) as key components of tertiary lymphoid structures and plasma cells made up high percentages of the lymphocyte infiltrate. Percentages of regulatory B cell varied depending on the regulatory phenotype. Analysis of humoral immune responses against 23 tumor-associated antigens (TAA) showed reactivity against at least one antigen in 56% of HNSCC patients. Reactivity was less frequent in human papillomavirus associated (HPV+) patients and healthy controls compared to HPV negative (HPV-) HNSCC. Likewise, patients with early stage HNSCC or MHC-I loss on tumor cells had low TAA responses. Patients with TAA responses showed CD4(+) dominated T cell infiltration compared to mainly CD8(+) T cells in tumors without detected TAA response. To summarize, our data demonstrates different immune infiltration patterns in relation to serological TAA response detection and the presence of B cell subpopulations in HNSCC that can engage in tumor promoting and antitumor activity. In view of increasing use of immunotherapeutic approaches, it will be important to include B cells into comprehensive phenotypic and functional analyses of tumor-associated lymphocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lechner, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Schloesser, Hans A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Thelen, Martin UNSPECIFIED orcid.org/0000-0002-2785-9726 UNSPECIFIED Wennhold, Kerstin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothschild, Sacha, I UNSPECIFIED UNSPECIFIED UNSPECIFIED Gilles, Ramona UNSPECIFIED UNSPECIFIED UNSPECIFIED Quaas, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Siefer, Oliver G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huebbers, Christian U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cukuroglu, Engin UNSPECIFIED UNSPECIFIED UNSPECIFIED Goeke, Jonathan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hillmer, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Gathof, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Moritz F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Klussmann, Jens P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shimabukuro-Vornhagen, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Theurich, Sebastian UNSPECIFIED orcid.org/0000-0001-5706-8258 UNSPECIFIED Beutner, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED von Bergwelt-Baildon, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-139856
DOI:	10.1080/2162402X.2018.1535293
Journal or Publication Title:	OncoImmunology
Volume:	8
Number:	3
Date:	2019
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	2162-402X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CANCER-TESTIS ANTIGENS; TERTIARY LYMPHOID STRUCTURES; REGULATORY T-CELLS; CLASS-I EXPRESSION; PLASMA-CELLS; SURVIVAL; PHENOTYPE; INFILTRATION; LYMPHOCYTES; MARKER Multiple languages Oncology; Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13985