Universität zu Köln

Xu, Yan, Wang, Sibo, Li, Linzhou, Sahu, Sunil Kumar ORCID: 0000-0002-4742-9870, Petersen, Morten ORCID: 0000-0002-3035-5991, Liu, Xin ORCID: 0000-0003-3256-2940, Melkonian, Michael, Zhang, Gengyun and Liu, Huan ORCID: 0000-0003-3909-0931 (2019). Molecular evidence for origin, diversification and ancient gene duplication of plant subtilases (SBTs). Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Plant subtilases (SBTs) are a widely distributed family of serine proteases which participates in plant developmental processes and immune responses. Although SBTs are divided into seven subgroups in plants, their origin and evolution, particularly in green algae remain elusive. Here, we present a comprehensive large-scale evolutionary analysis of all subtilases. The plant subtilases SBT1-5 were found to be monophyletic, nested within a larger radiation of bacteria suggesting that they originated from bacteria by a single horizontal gene transfer (HGT) event. A group of bacterial subtilases comprising representatives from four phyla was identified as a sister group to SBT1-5. The phylogenetic analyses, based on evaluation of novel streptophyte algal genomes, suggested that the recipient of the HGT of bacterial subtilases was the common ancestor of Coleochaetophyceae, Zygnematophyceae and embryophytes. Following the HGT, the subtilase gene duplicated in the common ancestor and the two genes diversified into SBT2 and SBT1, 3-5 respectively. Comparative structural analysis of homology-modeled SBT2 proteins also showed their conservation from bacteria to embryophytes. Our study provides the first molecular evidence about the evolution of plant subtilases via HGT followed by a first gene duplication in the common ancestor of Coleochaetophyceae, Zygnematophyceae, and embryophytes, and subsequent expansion in embryophytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xu, Yan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Sibo UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Linzhou UNSPECIFIED UNSPECIFIED UNSPECIFIED Sahu, Sunil Kumar UNSPECIFIED orcid.org/0000-0002-4742-9870 UNSPECIFIED Petersen, Morten UNSPECIFIED orcid.org/0000-0002-3035-5991 UNSPECIFIED Liu, Xin UNSPECIFIED orcid.org/0000-0003-3256-2940 UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Gengyun UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Huan UNSPECIFIED orcid.org/0000-0003-3909-0931 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143853
DOI:	10.1038/s41598-019-48664-6
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYSTAL-STRUCTURE; STRESS RESPONSES; STRUCTURAL BASIS; SERINE-PROTEASE; SUBTILISIN; CUCUMISIN; PEPTIDASES; FAMILY Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14385