Vogel, Stefanie, Schaefer, Cordula, Hess, Simon, Folz-Donahue, Kat, Nelles, Melanie, Minassian, Anuka, Schwarz, Martin K., Kukat, Christian ORCID: 0000-0003-1508-0229, Ehrlich, Marc, Zaehres, Holm, Kloppenburg, Peter, Hoehn, Mathias and Aswendt, Markus ORCID: 0000-0003-1423-0934 (2019). The in vivo timeline of differentiation of engrafted human neural progenitor cells. Stem Cell Res., 37. AMSTERDAM: ELSEVIER. ISSN 1876-7753

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Abstract

Understanding the individual timeline of stem cell differentiation in vivo is critical for evaluating stem cell properties in animal models. However, with conventional ex vivo techniques, such as histology, the individual timeline of differentiation is not accessible. Therefore, we designed lentiviral plasmids with cell-specific promoters to control the expression of bioluminescence and fluorescence imaging reporters. Promoter-dependent reporter expression in transduced human induced pluripotent stem cell-derived neural progenitor cells (hNPCs) was an effective indicator of differentiation in cell culture. A 12-week in vivo imaging observation period revealed the time profile of differentiation of engrafted hNPCs in the mouse brain into astrocytes and mature neurons which was verified by immunostainings, patch-clamp electrophysiology, and light-sheet fluorescence microscopy. The lentiviral vectors validated in this study provide an efficient imaging toolbox for non-invasive and longitudinal characterization of stem cell differentiation, in vitro screenings, and in vivo studies of cell therapy in animal models.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Vogel, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaefer, CordulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hess, SimonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Folz-Donahue, KatUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nelles, MelanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Minassian, AnukaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schwarz, Martin K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kukat, ChristianUNSPECIFIEDorcid.org/0000-0003-1508-0229UNSPECIFIED
Ehrlich, MarcUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zaehres, HolmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kloppenburg, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoehn, MathiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aswendt, MarkusUNSPECIFIEDorcid.org/0000-0003-1423-0934UNSPECIFIED
URN: urn:nbn:de:hbz:38-148911
DOI: 10.1016/j.scr.2019.101429
Journal or Publication Title: Stem Cell Res.
Volume: 37
Date: 2019
Publisher: ELSEVIER
Place of Publication: AMSTERDAM
ISSN: 1876-7753
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
STEM-CELLS; FUNCTIONAL RECOVERY; BRAIN; ASTROCYTES; STROKE; EXPRESSION; TRANSPLANTATION; PROFILES; PLATFORM; PROTEINMultiple languages
Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/14891

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