Wang, Jizong ORCID: 0000-0003-1085-5347, Wang, Jia ORCID: 0000-0002-5978-4191, Hu, Meijuan ORCID: 0000-0001-5326-0816, Wu, Shan ORCID: 0000-0002-4736-4755, Qi, Jinfeng ORCID: 0000-0002-4660-7495, Wang, Guoxun ORCID: 0000-0002-8033-5980, Han, Zhifu, Qi, Yijun, Gao, Ning ORCID: 0000-0003-3067-9993, Wang, Hong-Wei, Zhou, Jian-Min ORCID: 0000-0002-9943-2975 and Chai, Jijie (2019). Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364 (6435). S. 43 - 81. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2(UMP)) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo-electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2(UMP) in an inactive and intermediate state, respectively. The ZAR1(LRR) domain, compared with animal NLRLRR domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2(UMP) is exclusively through RKS1, which interacts with ZAR1(LRR). PBL2(UMP) binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wang, JizongUNSPECIFIEDorcid.org/0000-0003-1085-5347UNSPECIFIED
Wang, JiaUNSPECIFIEDorcid.org/0000-0002-5978-4191UNSPECIFIED
Hu, MeijuanUNSPECIFIEDorcid.org/0000-0001-5326-0816UNSPECIFIED
Wu, ShanUNSPECIFIEDorcid.org/0000-0002-4736-4755UNSPECIFIED
Qi, JinfengUNSPECIFIEDorcid.org/0000-0002-4660-7495UNSPECIFIED
Wang, GuoxunUNSPECIFIEDorcid.org/0000-0002-8033-5980UNSPECIFIED
Han, ZhifuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Qi, YijunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gao, NingUNSPECIFIEDorcid.org/0000-0003-3067-9993UNSPECIFIED
Wang, Hong-WeiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, Jian-MinUNSPECIFIEDorcid.org/0000-0002-9943-2975UNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-150944
DOI: 10.1126/science.aav5868
Journal or Publication Title: Science
Volume: 364
Number: 6435
Page Range: S. 43 - 81
Date: 2019
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 1095-9203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CRYO-EM STRUCTURE; RUST RESISTANCE PROTEIN; CRYSTAL-STRUCTURE; STRUCTURAL BASIS; RHO GTPASES; GENE; REVEALS; RECOGNITION; APOPTOSOME; EFFECTORMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15094

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