Wang, Jizong ORCID: 0000-0003-1085-5347, Hu, Meijuan ORCID: 0000-0001-5326-0816, Wang, Jia ORCID: 0000-0002-5978-4191, Qi, Jinfeng ORCID: 0000-0002-4660-7495, Han, Zhifu, Wang, Guoxun ORCID: 0000-0002-8033-5980, Qi, Yijun, Wang, Hong-Wei, Zhou, Jian-Min ORCID: 0000-0002-9943-2975 and Chai, Jijie (2019). Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364 (6435). S. 44 - 93. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an a-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic a helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wang, JizongUNSPECIFIEDorcid.org/0000-0003-1085-5347UNSPECIFIED
Hu, MeijuanUNSPECIFIEDorcid.org/0000-0001-5326-0816UNSPECIFIED
Wang, JiaUNSPECIFIEDorcid.org/0000-0002-5978-4191UNSPECIFIED
Qi, JinfengUNSPECIFIEDorcid.org/0000-0002-4660-7495UNSPECIFIED
Han, ZhifuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, GuoxunUNSPECIFIEDorcid.org/0000-0002-8033-5980UNSPECIFIED
Qi, YijunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, Hong-WeiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, Jian-MinUNSPECIFIEDorcid.org/0000-0002-9943-2975UNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-150957
DOI: 10.1126/science.aav5870
Journal or Publication Title: Science
Volume: 364
Number: 6435
Page Range: S. 44 - 93
Date: 2019
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 1095-9203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CRYO-EM STRUCTURE; RUST RESISTANCE PROTEIN; DOMAIN-LIKE PROTEIN; DISEASE RESISTANCE; CELL-DEATH; CRYSTAL-STRUCTURE; COILED-COIL; CASPASE-9 ACTIVATION; PATHOGEN PERCEPTION; NUCLEOTIDE-BINDINGMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15095

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