Universität zu Köln

Rossen, John W. A., Dombrecht, Jill, Vanfleteren, Diederik, De Bruyne, Katrien, van Belkum, Alex, Rosema, Sigrid, Lokate, Mariette, Bathoorn, Erik, Reuter, Sandra, Grundmann, Hajo, Ertel, Julia, Higgins, Paul G. ORCID: 0000-0001-8677-9454 and Seifert, Harald (2019). Epidemiological Typing of Serratia marcescens Isolates by Whole-Genome Multilocus Sequence Typing. J. Clin. Microbiol., 57 (4). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

Serratia marcescens is an opportunistic bacterial pathogen. It is notorious for its increasing antimicrobial resistance and its potential to cause outbreaks of colonization and infections, predominantly in neonatal intensive care units (NICUs). There, its spread requires rapid infection control response. To understand its spread, detailed molecular typing is key. We present a whole-genome multilocus sequence typing (wgMLST) method for S. marcescens. Using a set of 299 publicly available whole-genome sequences (WGS), we developed an initial wgMLST system consisting of 9,377 gene loci. This included 1,455 loci occurring in all reference genomes and 7,922 accessory loci. This closed system was validated using three geographically diverse collections of S. marcescens consisting of 111 clinical isolates implicated in nosocomial dissemination events in three hospitals. The validation procedure showed a full match between epidemiological data and the wgMLST analyses. We set the cut- off value for epidemiological (non)relatedness at 20 different alleles, though for the majority of outbreak-clustered isolates, this difference was limited to 4 alleles. This shows that the wgMLST system for S. marcescens provides prospects for successful future monitoring for the epidemiological containment of this opportunistic pathogen.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rossen, John W. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dombrecht, Jill UNSPECIFIED UNSPECIFIED UNSPECIFIED Vanfleteren, Diederik UNSPECIFIED UNSPECIFIED UNSPECIFIED De Bruyne, Katrien UNSPECIFIED UNSPECIFIED UNSPECIFIED van Belkum, Alex UNSPECIFIED UNSPECIFIED UNSPECIFIED Rosema, Sigrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Lokate, Mariette UNSPECIFIED UNSPECIFIED UNSPECIFIED Bathoorn, Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Reuter, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Grundmann, Hajo UNSPECIFIED UNSPECIFIED UNSPECIFIED Ertel, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-152492
DOI:	10.1128/JCM.01652-18
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	57
Number:	4
Date:	2019
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTENSIVE-CARE-UNIT; HOSPITAL HYGIENE; OUTBREAK; DISEASE; INFECTIONS Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15249