Frank, Stefan, Ahuja, Gaurav ORCID: 0000-0002-2837-9361, Bartsch, Deniz, Russ, Nicole, Yao, Wenjie, Kuo, Joseph Chao-Chung, Derks, Jens-Peter, Akhade, Vijay Suresh, Kargapolova, Yulia, Georgomanolis, Theodore, Messling, Jan-Erik ORCID: 0000-0003-3843-0041, Gramm, Marie, Brant, Lilija, Rehimi, Rizwan, Vargas, Natalia Emilse, Kuroczik, Alina, Yang, Tsun-Po, Sahito, Raja Ghazanfar Ali, Franzen, Julia, Hescheler, Juergen, Sachinidis, Agapios, Peifer, Martin, Rada-Iglesias, Alvaro ORCID: 0000-0001-7137-1341, Kanduri, Meena, Costa, Ivan G. ORCID: 0000-0003-2890-8697, Kanduri, Chandrasekhar, Papantonis, Argyris ORCID: 0000-0001-7551-1073 and Kurian, Leo ORCID: 0000-0001-7466-5169 (2019). yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs. Cell Stem Cell, 24 (2). S. 318 - 336. CAMBRIDGE: CELL PRESS. ISSN 1875-9777

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Abstract

Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Frank, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ahuja, GauravUNSPECIFIEDorcid.org/0000-0002-2837-9361UNSPECIFIED
Bartsch, DenizUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Russ, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yao, WenjieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuo, Joseph Chao-ChungUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Derks, Jens-PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Akhade, Vijay SureshUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kargapolova, YuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Georgomanolis, TheodoreUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Messling, Jan-ErikUNSPECIFIEDorcid.org/0000-0003-3843-0041UNSPECIFIED
Gramm, MarieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brant, LilijaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rehimi, RizwanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vargas, Natalia EmilseUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuroczik, AlinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yang, Tsun-PoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sahito, Raja Ghazanfar AliUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franzen, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sachinidis, AgapiosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peifer, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rada-Iglesias, AlvaroUNSPECIFIEDorcid.org/0000-0001-7137-1341UNSPECIFIED
Kanduri, MeenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Costa, Ivan G.UNSPECIFIEDorcid.org/0000-0003-2890-8697UNSPECIFIED
Kanduri, ChandrasekharUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Papantonis, ArgyrisUNSPECIFIEDorcid.org/0000-0001-7551-1073UNSPECIFIED
Kurian, LeoUNSPECIFIEDorcid.org/0000-0001-7466-5169UNSPECIFIED
URN: urn:nbn:de:hbz:38-156848
DOI: 10.1016/j.stem.2018.11.005
Journal or Publication Title: Cell Stem Cell
Volume: 24
Number: 2
Page Range: S. 318 - 336
Date: 2019
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1875-9777
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GENE-EXPRESSION; NONCODING RNA; DIFFERENTIATION; INDUCTION; BRACHYURY; DYNAMICS; LINCRNASMultiple languages
Cell & Tissue Engineering; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15684

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