Rinschen, Markus M. (2019). Single glomerular proteomics: A novel tool for translational glomerular cell biology. In: Methods in Cell Biology, S. 1 - 6. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC. ISBN 978-0-12-820336-1; 978-0-12-820335-4
Full text not available from this repository.Abstract
The glomerulus harbors the renal filtration barrier and needs to be precisely maintained. In this chapter, the concept of single glomerular proteomics is described. Single glomerular proteomics has recently been enabled by advances in glomerular isolation, ultrasensitive peptide sample preparation and mass spectrometry based technology and acquisition strategies. It generates protein content information on a single glomerulus that can be overlaid with morphological and other multi-layered omics analyses. The novel method consists of four essential steps: preparation of single glomeruli-by microdissection, glomerular preparation, or laser microdissection-followed by proteomic sample preparation, mass spectrometry analysis and bioinformatics analysis. It enables for the first time the generation of sub-biopsy level proteomics data. In perspective, comprehensive data from individual glomeruli could be used in order to pinpoint novel druggable targets in animal models of kidney disease or in patients with proteinuria and glomerular disease.
| Item Type: | Book Section, Proceedings Item or annotation in a legal commentary | ||||||||
| Creators: |
|
||||||||
| URN: | urn:nbn:de:hbz:38-160245 | ||||||||
| DOI: | 10.1016/bs.mcb.2019.03.010 | ||||||||
| Title of Book: | Methods in Cell Biology | ||||||||
| Series Name: | Methods Cell Biol. | ||||||||
| Volume: | 154 | ||||||||
| Page Range: | S. 1 - 6 | ||||||||
| Date: | 2019 | ||||||||
| Publisher: | ELSEVIER ACADEMIC PRESS INC | ||||||||
| Place of Publication: | SAN DIEGO | ||||||||
| ISSN: | 0091-679X | ||||||||
| ISBN: | 978-0-12-820336-1; 978-0-12-820335-4 | ||||||||
| Language: | English | ||||||||
| Faculty: | Unspecified | ||||||||
| Divisions: | Unspecified | ||||||||
| Subjects: | no entry | ||||||||
| Uncontrolled Keywords: |
|
||||||||
| Refereed: | Yes | ||||||||
| URI: | http://kups.ub.uni-koeln.de/id/eprint/16024 |
Downloads
Downloads per month over past year
Altmetric
Export
Actions (login required)
 |
View Item |