Reichel, Christian, Gmeiner, Guenter, Walpurgis, Katja and Thevis, Mario (2018). Updated protocols for the detection of Sotatercept and Luspatercept in human serum. Drug Test. Anal., 10 (11-12). S. 1708 - 1714. HOBOKEN: WILEY. ISSN 1942-7611

Full text not available from this repository.

Abstract

We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively high amount of antibody required per sample (10 mu g) and the need of a secondary antibody for the final detection. The updated protocols overcome these limitations by antigen-antibody complex formation in solution followed by capture of the complex with anti-antibody-coated magnetic beads. They also omit the secondary antibody incubation step by usage of biotinylated primary antibodies, which can be directly incubated with streptavidin-HRP. Thus, the new protocols are faster, simpler, and cheaper and offer comparable sensitivities.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Reichel, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gmeiner, GuenterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Walpurgis, KatjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-166555
DOI: 10.1002/dta.2500
Journal or Publication Title: Drug Test. Anal.
Volume: 10
Number: 11-12
Page Range: S. 1708 - 1714
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1942-7611
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ERYTHROPOIESIS; ACE-011; ACE-536; ACTRIIA-IGG1; ANEMIA; AGENT; PAGEMultiple languages
Biochemical Research Methods; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/16655

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item