Reichel, Christian, Gmeiner, Guenter, Walpurgis, Katja and Thevis, Mario (2018). Updated protocols for the detection of Sotatercept and Luspatercept in human serum. Drug Test. Anal., 10 (11-12). S. 1708 - 1714. HOBOKEN: WILEY. ISSN 1942-7611
Full text not available from this repository.Abstract
We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively high amount of antibody required per sample (10 mu g) and the need of a secondary antibody for the final detection. The updated protocols overcome these limitations by antigen-antibody complex formation in solution followed by capture of the complex with anti-antibody-coated magnetic beads. They also omit the secondary antibody incubation step by usage of biotinylated primary antibodies, which can be directly incubated with streptavidin-HRP. Thus, the new protocols are faster, simpler, and cheaper and offer comparable sensitivities.
Item Type: | Journal Article | ||||||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-166555 | ||||||||||||||||||||
DOI: | 10.1002/dta.2500 | ||||||||||||||||||||
Journal or Publication Title: | Drug Test. Anal. | ||||||||||||||||||||
Volume: | 10 | ||||||||||||||||||||
Number: | 11-12 | ||||||||||||||||||||
Page Range: | S. 1708 - 1714 | ||||||||||||||||||||
Date: | 2018 | ||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||
ISSN: | 1942-7611 | ||||||||||||||||||||
Language: | English | ||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||
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Refereed: | Yes | ||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/16655 |
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