Brüx, Christian (2005). Strukturen der beta-D-Xylosidase aus Geobacillus stearothermophilus T-6 in verschiedenen kristallinen und funktionellen Zuständen. PhD thesis, Universität zu Köln.

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Abstract

In dieser Arbeit wird die Struktur der beta-Xylosidase aus Geobacillus stearothermophilus vorgestellt. Die Xylosidase gehört zur Glykosid-Hydrolase Familie 43. Sie zeigt ein fünf flügelige beta-Propeller Faltung. Darüber hinaus werden die Strukturen von drei katalytisch inaktiven Punktmutanten, jeweils kokristallisiert mit den natürlichen Substrat Xylobiose, beschrieben. Die daraus gewonnenen Erkenntnisse erlauben eine strukturelle Erklärung der Quartärstruktur, Reaktionsmechanismus und Spezifität des Enzyms.

Item Type: Thesis (PhD thesis)
Translated title:
Title
Language
Structures of the beta-D-Xylosidase from Geobacillus stearothermophilus in different crystalline and functional state
English
Translated abstract:
Abstract
Language
Here we present the structure of the beta-xylosidase from Geobacillus stearothermophilus. This xylosidase is part of the glycoside hydrolase family 43. It shows a five blade beta propeller fold. Further, the structures of three catalytic inactive point mutations, each cocrystallized with the natural substrate xylobiose, are described. These structures allow a structural understanding of quarternary structure, reaction mechanism and substrate specificity of the enzyme.
English
Creators:
Creators
Email
ORCID
ORCID Put Code
Brüx, Christian
acc37@uni-koeln.de
UNSPECIFIED
UNSPECIFIED
URN: urn:nbn:de:hbz:38-16865
Date: 2005
Language: German
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects: Life sciences
Uncontrolled Keywords:
Keywords
Language
xylosidase , xylose , hemicellulose , struktur , glykosidase
German
xylosidase , structure , glycosidase , xylose
English
Date of oral exam: 6 February 2006
Referee:
Name
Academic Title
Schomburg, Dietmar
Prof. Dr.
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/1686

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