Universität zu Köln

Kirn, Verena, Strake, Leonie, Thangarajah, Fabinshy, Richters, Lisa, Eischeid, Hannah, Koitzsch, Ulrike, Odenthal, Margarete and Fries, Jochen ORCID: 0000-0003-2054-6345 (2018). ESR1-promoter-methylation status in primary breast cancer and its corresponding metastases. Clin. Exp. Metastasis, 35 (7). S. 707 - 713. DORDRECHT: SPRINGER. ISSN 1573-7276

Full text not available from this repository.

Abstract

The role of ESR1 methylation in breast cancer and its influence on disease progression is not yet fully understood. Healthy breast tissue usually does not show ESR1 promoter methylation, whereas the frequency of ESR1 methylation appears to increase in primary breast cancer and in metastatic disease. Although women with ER positive breast cancer have a good prognosis, some will relapse. We aimed to evaluate the methylation status of ESR1 in primary breast cancer and its corresponding metastases by a methylation-specific real-time PCR and to correlate the methylation status with clinical outcome. Women who were treated for primary and metastatic breast cancer were included in the study. Tumor DNA was isolated from paraffin embedded tissue sections. After bisulfite treatment ESR1 promoter methylation was analyzed by real time-MSP of each tissue sample. Kaplan-Meier-Curves were drawn for survival. In the group of patients with positive ESR1 promoter methylation in the primary breast carcinoma survival was lower compared to the group of patients without methylation (38.1months vs. 54.3months, n.s.). Seven out of 19 (37%) of those patients with positive ESR1 promoter methylation developed loss of ER expression in metastatic disease. None of the patients who had primary tumours that were ESR1 methylation negative developed ER expression negative metastatic disease. The results underline the importance of the ESR1 promoter methylation and its potential application as a predictive marker. To improve the clinical outcome of patients with metastatic disease, those with initially positive ESR1 methylation status should undergo a tissue biopsy already at the beginning of metastatic disease to identify those with loss of ER expression and thus resitance to anti-endocrine therapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kirn, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Strake, Leonie UNSPECIFIED UNSPECIFIED UNSPECIFIED Thangarajah, Fabinshy UNSPECIFIED UNSPECIFIED UNSPECIFIED Richters, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Eischeid, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Koitzsch, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Odenthal, Margarete UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen UNSPECIFIED orcid.org/0000-0003-2054-6345 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-171060
DOI:	10.1007/s10585-018-9935-5
Journal or Publication Title:	Clin. Exp. Metastasis
Volume:	35
Number:	7
Page Range:	S. 707 - 713
Date:	2018
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-7276
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RECEPTOR GENE-EXPRESSION; PROGESTERONE-RECEPTOR; DNA METHYLATION; ESTROGEN-RECEPTOR; ESR1; HYPERMETHYLATION; TUMORS; SERUM Multiple languages Oncology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17106