Universität zu Köln

Baldin, Clara, Soliman, Sameh S. M., Jeon, Heewon H., Alkhazraji, Sondus, Gebremariam, Teclegiorgis, Gu, Yiyou, Bruno, Vincent M., Cornely, Oliver A., Leather, Helen L., Sugrue, Michele W., Wingard, John R., Stevens, David A., Edwards, John E., Jr. and Ibrahim, Ashraf S. (2018). PCR-Based Approach Targeting Mucorales-Specific Gene Family for Diagnosis of Mucormycosis. J. Clin. Microbiol., 56 (10). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

Mucormycosis is an aggressive, life-threatening infection caused by fungi in the order Mucorales. The current diagnosis of mucormycosis relies on mycological cultures, radiology and histopathology. These methods lack sensitivity and are most definitive later in the course of infection, resulting in the prevention of timely intervention. PCR-based approaches have shown promising potential in rapidly diagnosing mucormycosis. The spore coating protein homolog encoding CotH genes are uniquely and universally present among Mucorales. Thus, CotH genes are potential targets for the rapid diagnosis of mucormycosis. We infected mice with different Mucorales known to cause human mucormycosis and investigated whether CotH could be PCR amplified from biological fluids. Uninfected mice and those with aspergillosis were used to determine the specificity of the assay. CotH was detected as early as 24 h postinfection in plasma, urine, and bronchoalveolar lavage (BAL) samples from mice infected intratracheally with Rhizopus delemar, Rhizopus oryzae, Mucor circinelloides, Lichtheimia corymbifera, or Cunninghamella bertholletiae but not from samples taken from uninfected mice or mice infected with Aspergillus fumigatus. Detection of CotH from urine samples was more reliable than from plasma or BAL fluid. Using the receiver operating characteristic method, the sensitivity and the specificity of the assay were found to be 90 and 100%, respectively. Finally, CotH was PCR amplified from urine samples of patients with proven mucormycosis. Thus, PCR amplification of CotH is a promising target for the development of a reliable, sensitive, and simple method of early diagnosis of mucormycosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Baldin, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Soliman, Sameh S. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeon, Heewon H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Alkhazraji, Sondus UNSPECIFIED UNSPECIFIED UNSPECIFIED Gebremariam, Teclegiorgis UNSPECIFIED UNSPECIFIED UNSPECIFIED Gu, Yiyou UNSPECIFIED UNSPECIFIED UNSPECIFIED Bruno, Vincent M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leather, Helen L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sugrue, Michele W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wingard, John R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stevens, David A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Edwards, John E., Jr. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ibrahim, Ashraf S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-171606
DOI:	10.1128/JCM.00746-18
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	56
Number:	10
Date:	2018
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIPOSOMAL AMPHOTERICIN-B; BRONCHOALVEOLAR LAVAGE; MURINE MODEL; CUTANEOUS MUCORMYCOSIS; CLINICAL MICROBIOLOGY; MOLD INFECTIONS; RAPID DETECTION; DNA DETECTION; ZYGOMYCOSIS; IDENTIFICATION Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17160