Universität zu Köln

Bowe, Andrea, Zweerink, Susanne ORCID: 0000-0003-0926-8436, Mueck, Vera, Kondylis, Vangelis ORCID: 0000-0002-6970-8731, Schulte, Sigrid, Goeser, Tobias and Nierhoff, Dirk (2018). Depolarized Hepatocytes Express the Stem/Progenitor Cell Marker Neighbor of Punc E11 After Bile Duct Ligation in Mice. J. Histochem. Cytochem., 66 (8). S. 563 - 577. LONDON: SAGE PUBLICATIONS LTD. ISSN 1551-5044

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Abstract

There is a medical need of biomarkers for disease stratification in cholestatic liver diseases that come along with changes in hepatocyte polarity. Neighbor of Punc E11 (Nope) is an oncofetal marker that is lost after final differentiation and polarization of hepatocytes. We analyzed the expression pattern of Nope and connexin (Cx) 26 as markers of hepatocyte polarization during murine liver development as well as in adult liver with or without bile duct ligation (BDL) by quantitative real-time reverse transcription polymerise chain reaction (qRT-PCR), western blotting (WB), and immunohistochemistry. Nope is highly expressed in fetal and postnatal liver but barely detectable thereafter. Cx26, however, is much higher expressed in adult than in fetal liver. Postnatally, Nope is directed to the sinusoidal membrane of early hepatocytes while Cx26 remains distributed over the whole membrane indicating limited polarization. In the adult liver, only Cx26 is detectable and restricted to the bile canalicular domain indicating fully polarized hepatocytes. After BDL, Nope is again >300-fold upregulated while Cx26 is reduced rapidly. By immunohistochemistry, Nope identifies a subset of hepatocytes with randomly distributed Cx26. In summary, Nope identifies depolarized adult hepatocytes after cholestatic liver injury resembling early postnatal hepatocytes. Therefore, Nope might be a valuable histochemical biomarker allowing stage-specific stratifications in cholestatic liver diseases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bowe, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Zweerink, Susanne UNSPECIFIED orcid.org/0000-0003-0926-8436 UNSPECIFIED Mueck, Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED Kondylis, Vangelis UNSPECIFIED orcid.org/0000-0002-6970-8731 UNSPECIFIED Schulte, Sigrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Goeser, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Nierhoff, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-177644
DOI:	10.1369/0022155418768230
Journal or Publication Title:	J. Histochem. Cytochem.
Volume:	66
Number:	8
Page Range:	S. 563 - 577
Date:	2018
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1551-5044
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAP JUNCTIONAL CHANNELS; REGENERATING RAT-LIVER; INTERCELLULAR COMMUNICATION; HEPATOCELLULAR-CARCINOMA; GUIDANCE RECEPTORS; NEOGENIN; POLARITY; FETAL; GENE; CONNEXINS Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17764