Universität zu Köln

Krug, Oliver, Thomas, Andreas ORCID: 0000-0003-1199-0743, Malerod-Fjeld, Helle, Dehnes, Yvette, Laussmann, Tim ORCID: 0000-0002-0180-3513, Feldmann, Ingo, Sickmann, Albert ORCID: 0000-0002-2388-5265 and Thevis, Mario (2018). Analysis of new growth promoting black market products. Growth Horm. IGF Res., 41. S. 1 - 7. EDINBURGH: CHURCHILL LIVINGSTONE. ISSN 1532-2238

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Abstract

Detecting agents allegedly or evidently promoting growth such as human growth hormone (GH) or growth hormone releasing peptides (GHRP) in doping controls has represented a pressing issue for sports drug testing laboratories. While GH is a recombinant protein with a molecular weight of 22 kDa, the GHRPs are short (3-6 amino acids long) peptides with GH releasing properties. The endogenously produced GH (22 kDa isoform) consists of 191 amino acids and has a monoisotopic molecular mass of 22,124 Da. Within this study, a slightly modified form of GH was discovered consisting of 192 amino acids carrying an additional alanine at the N terminus, leading to a monoisotopic mass of 22,195 Da. This was confirmed by top-down and bottom-up experiments using liquid chromatography coupled to high resolution/high accuracy mass spectrometry. Additionally, three analogues of GHRPs were identified as Gly-GHRP-6, Gly-GHRP-2 and Gly-Ipamorelin, representing the corresponding GHRP extended by a N-terminal glycine residue. The structure of these peptides was characterised by means of high resolution (tandem) mass spectrometry, and for Gly-Ipamorelin and Gly-GHRP-2 their identity was additionally confirmed by custom synthesis. Further, established in-vitro experiments provided preliminary information considering the potential metabolism after administration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Krug, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Malerod-Fjeld, Helle UNSPECIFIED UNSPECIFIED UNSPECIFIED Dehnes, Yvette UNSPECIFIED UNSPECIFIED UNSPECIFIED Laussmann, Tim UNSPECIFIED orcid.org/0000-0002-0180-3513 UNSPECIFIED Feldmann, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Sickmann, Albert UNSPECIFIED orcid.org/0000-0002-2388-5265 UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-178241
DOI:	10.1016/j.ghir.2018.05.001
Journal or Publication Title:	Growth Horm. IGF Res.
Volume:	41
Page Range:	S. 1 - 7
Date:	2018
Publisher:	CHURCHILL LIVINGSTONE
Place of Publication:	EDINBURGH
ISSN:	1532-2238
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HORMONE-RELEASING PEPTIDES; SPORT; INSULIN Multiple languages Cell Biology; Endocrinology & Metabolism Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17824