Jacoby, Richard P., Martyn, Anna and Kopriva, Stanislav ORCID: 0000-0002-7416-6551 (2018). Exometabolomic Profiling of Bacterial Strains as Cultivated Using Arabidopsis Root Extract as the Sole Carbon Source. Mol. Plant-Microbe Interact., 31 (8). S. 803 - 814. ST PAUL: AMER PHYTOPATHOLOGICAL SOC. ISSN 1943-7706

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Abstract

The ability of microorganisms to use root-derived metabolites as growth substrates is a key trait for success in the rhizospheric niche. However, few studies describe which specific metabolites are consumed or to what degree microbial strains differ in their substrate consumption patterns. Here, we present a liquid chromatography-mass spectrometry (MS) exometabolomic study of three bacterial strains cultivated using either glucose or Arabidopsis thaliana root extract as the sole carbon source. Two of the strains were previously isolated from field-grown Arabidopsis roots, the other is Escherichia coli, included as a comparison. When cultivated on root extract, a set of 62 MS features were commonly taken up by all three strains, with m/z values matching components of central metabolism (including amino acids and purine or pyrimidine derivatives). Escherichia coli took up very few MS features outside this commonly consumed set, whereas the root-inhabiting strains took up a much larger number of MS features, many with m/z values matching plant-specific metabolites. These measurements define the metabolic niche that each strain potentially occupies in the rhizosphere. Furthermore, we document many MS features released by these strains that could play roles in cross-feeding, antibiosis, or signaling. We present our methodological approach as a foundation for future studies of rhizosphere exometabolomics.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Jacoby, Richard P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Martyn, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kopriva, StanislavUNSPECIFIEDorcid.org/0000-0002-7416-6551UNSPECIFIED
URN: urn:nbn:de:hbz:38-178948
DOI: 10.1094/MPMI-10-17-0253-R
Journal or Publication Title: Mol. Plant-Microbe Interact.
Volume: 31
Number: 8
Page Range: S. 803 - 814
Date: 2018
Publisher: AMER PHYTOPATHOLOGICAL SOC
Place of Publication: ST PAUL
ISSN: 1943-7706
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHROMATOGRAPHY-MASS SPECTROMETRY; MICROBIAL-GROWTH; RHIZOSPHERE; METABOLOMICS; PLANTS; RHIZODEPOSITS; ENDOPHYTES; PURPOSE; WILD; FLUXMultiple languages
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/17894

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