Singh, Indrabahadur ORCID: 0000-0002-1474-7928, Contreras, Adriana, Cordero, Julio, Rubio, Karla ORCID: 0000-0002-8337-3106, Dobersch, Stephanie, Guenther, Stefan, Jeratsch, Sylvia, Mehta, Aditi, Krueger, Marcus, Graumann, Johannes ORCID: 0000-0002-3015-5850, Seeger, Werner ORCID: 0000-0003-1946-0894, Dobreva, Gergana ORCID: 0000-0002-4814-9416, Braun, Thomas ORCID: 0000-0002-6165-4804 and Barreto, Guillermo ORCID: 0000-0002-7777-4712 (2018). MiCEE is a ncRNA-protein complex that mediates epigenetic silencing and nucleolar organization. Nature Genet., 50 (7). S. 990 - 1005. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1546-1718

Full text not available from this repository.

Abstract

The majority of the eukaryotic genome is transcribed into noncoding RNAs (ncRNAs), which are important regulators of different nuclear processes by controlling chromatin structure. However, the full extent of ncRNA function has remained elusive. Here we deciphered the function of the microRNA Mirlet7d as a key regulator of bidirectionally transcribed genes. We found that nuclear Mirlet7d binds ncRNAs expressed from these genes. Mirlet7d-ncRNA duplexes are further bound by C1D, which in turn targets the RNA exosome complex and the polycomb repressive complex 2 (PRC2) to the bidirectionally active loci. The exosome degrades the ncRNAs, whereas PRC2 induces heterochromatin and transcriptional silencing through EZH2. Moreover, this multicomponent RNA-protein complex, which we named MiCEE, tethers the regulated genes to the perinucleolar region and thus is required for proper nucleolar organization. Our study demonstrates that the MiCEE complex mediates epigenetic silencing of bidirectionally expressed genes and global genome organization.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Singh, IndrabahadurUNSPECIFIEDorcid.org/0000-0002-1474-7928UNSPECIFIED
Contreras, AdrianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cordero, JulioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rubio, KarlaUNSPECIFIEDorcid.org/0000-0002-8337-3106UNSPECIFIED
Dobersch, StephanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guenther, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jeratsch, SylviaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mehta, AditiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Graumann, JohannesUNSPECIFIEDorcid.org/0000-0002-3015-5850UNSPECIFIED
Seeger, WernerUNSPECIFIEDorcid.org/0000-0003-1946-0894UNSPECIFIED
Dobreva, GerganaUNSPECIFIEDorcid.org/0000-0002-4814-9416UNSPECIFIED
Braun, ThomasUNSPECIFIEDorcid.org/0000-0002-6165-4804UNSPECIFIED
Barreto, GuillermoUNSPECIFIEDorcid.org/0000-0002-7777-4712UNSPECIFIED
URN: urn:nbn:de:hbz:38-180594
DOI: 10.1038/s41588-018-0139-3
Journal or Publication Title: Nature Genet.
Volume: 50
Number: 7
Page Range: S. 990 - 1005
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: NEW YORK
ISSN: 1546-1718
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RIBOSOMAL-RNA GENES; NONCODING RNA; READ ALIGNMENT; STEM-CELLS; TRANSCRIPTION; EXOSOME; MICRORNAS; HETEROCHROMATIN; DNA; ELEMENTSMultiple languages
Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18059

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item