Ingold, S., Dorum, G., Hanson, E., Berti, A., Branicki, W., Brito, P., Elsmore, P., Gettings, K. B., Giangasparo, F., Gross, T. E., Hansen, S., Hanssen, E. N., Kampmann, M. -L., Kayser, M., Laurent, F. -X., Morling, N., Mosquera-Miguel, A., Parson, W., Phillips, C., Porto, M. J., Pospiech, E., Roeder, A. D., Schneider, P. M., Johann, K. Schulze, Steffen, C. R., Syndercombe-Court, D., Trautmann, M., van den Berge, M., van der Gaag, K. J., Vannier, J., Verdoliva, V., Vidaki, A., Xavier, C., Ballantyne, J. and Haas, C. (2018). Body fluid identification using a targeted mRNA massively parallel sequencing approach - results of a EUROFORGEN/EDNAP collaborative exercise. Forensic Sci. Int.-Genet., 34. S. 105 - 116. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

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Abstract

In a previous study we presented an assay for targeted mRNA sequencing for the identification of human body fluids, optimised for the Illumina MiSeq/FGx MPS platform. This assay, together with an additional in-house designed assay for the Ion Torrent PGM/S5 platform, was the basis for a collaborative exercise within 17 EUROFORGEN and EDNAP laboratories, in order to test the efficacy of targeted mRNA sequencing to identify body fluids. The task was to analyse the supplied dried body fluid stains and, optionally, participants' own bona fide or mock casework samples of human origin, according to specified protocols. The provided primer pools for the Illumina MiSeq/FGx and the Ion Torrent PGM/S5 platforms included 33 and 29 body fluid specific targets, respectively, to identify blood, saliva, semen, vaginal secretion, menstrual blood and skin. The results demonstrated moderate to high count values in the body fluid or tissue of interest with little to no counts in non-target body fluids. There was some inter-laboratory variability in read counts, but overall the results of the laboratories were comparable in that highly expressed markers showed high read counts and less expressed markers showed lower counts. We performed a partial least squares (PLS) analysis on the data, where blood, menstrual blood, saliva and semen markers and samples clustered well. The results of this collaborative mRNA massively parallel sequencing (MPS) exercise support targeted mRNA sequencing as a reliable body fluid identification method that could be added to the repertoire of forensic MPS panels.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ingold, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dorum, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanson, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Berti, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Branicki, W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brito, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Elsmore, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gettings, K. B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Giangasparo, F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gross, T. E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hansen, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanssen, E. N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kampmann, M. -L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kayser, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Laurent, F. -X.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morling, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mosquera-Miguel, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parson, W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Phillips, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Porto, M. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pospiech, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roeder, A. D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Johann, K. SchulzeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Steffen, C. R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Syndercombe-Court, D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Trautmann, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van den Berge, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van der Gaag, K. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vannier, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Verdoliva, V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vidaki, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xavier, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ballantyne, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-186916
DOI: 10.1016/j.fsigen.2018.01.002
Journal or Publication Title: Forensic Sci. Int.-Genet.
Volume: 34
Page Range: S. 105 - 116
Date: 2018
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1878-0326
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RNA/DNA CO-ANALYSIS; MENSTRUAL BLOOD; MARKERS; STAINS; DNA; BIOMARKERS; SALIVA; SKINMultiple languages
Genetics & Heredity; Medicine, LegalMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18691

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