Universität zu Köln

Mayer, Michael ORCID: 0000-0002-7820-1393, Takegami, Shigehiko, Neumeier, Michael, Rink, Simone, Jacobi von Wangelin, Axel, Schulte, Silja, Vollmer, Moritz, Griesbeck, Axel G. ORCID: 0000-0002-6546-3482, Duerkop, Axel ORCID: 0000-0003-3007-6471 and Baeumner, Antje J. (2018). Electrochemiluminescence Bioassays with a Water-Soluble Luminol Derivative Can Outperform Fluorescence Assays. Angew. Chem.-Int. Edit., 57 (2). S. 408 - 412. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3773

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Abstract

The most efficient and commonly used electro-chemiluminescence (ECL) emitters are luminol, [Ru(bpy)(3)](2+), and derivatives thereof. Luminol stands out due to its low excitation potential, but applications are limited by its insolubility under physiological conditions. The water-soluble mcarboxy luminol was synthesized in 15% yield and exhibited high solubility under physiological conditions and afforded a four-fold ECL signal increase (vs. luminol). Entrapment in DNA-tagged liposomes enabled a DNA assay with a detection limit of 3.2 pmol L-1, which is 150 times lower than the corresponding fluorescence approach. This remarkable sensitivity gain and the low excitation potential establish m-carboxy luminol as a superior ECL probe with direct relevance to chemiluminescence and enzymatic bioanalytical approaches.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mayer, Michael UNSPECIFIED orcid.org/0000-0002-7820-1393 UNSPECIFIED Takegami, Shigehiko UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumeier, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Rink, Simone UNSPECIFIED UNSPECIFIED UNSPECIFIED Jacobi von Wangelin, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulte, Silja UNSPECIFIED UNSPECIFIED UNSPECIFIED Vollmer, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Griesbeck, Axel G. UNSPECIFIED orcid.org/0000-0002-6546-3482 UNSPECIFIED Duerkop, Axel UNSPECIFIED orcid.org/0000-0003-3007-6471 UNSPECIFIED Baeumner, Antje J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-199064
DOI:	10.1002/anie.201708630
Journal or Publication Title:	Angew. Chem.-Int. Edit.
Volume:	57
Number:	2
Page Range:	S. 408 - 412
Date:	2018
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3773
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Organic Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LIPOSOMES; CHEMILUMINESCENCE Multiple languages Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19906