Universität zu Köln

Rios-Barrera, L. D., Sigurbjoernsdottir, S., Baer, M. and Leptin, M. ORCID: 0000-0001-7097-348X (2017). Dual function for Tango1 in secretion of bulky cargo and in ER-Golgi morphology. Proc. Natl. Acad. Sci. U. S. A., 114 (48). S. E10389 - 10. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

Full text not available from this repository.

Abstract

Tango1 enables ER-to-Golgi trafficking of large proteins. We show here that loss of Tango1, in addition to disrupting protein secretion and ER/Golgi morphology, causes ER stress and defects in cell shape. We find that the previously observed dependence of smaller cargos on Tango1 is a secondary effect. If large cargos like Dumpy, which we identify as a Tango1 cargo, are removed from the cell, nonbulky proteins reenter the secretory pathway. Removal of blocking cargo also restores cell morphology and attenuates the ER-stress response. Thus, failures in the secretion of nonbulky proteins, ER stress, and defective cell morphology are secondary consequences of bulky cargo retention. By contrast, ER/Golgi defects in Tango1-depleted cells persist in the absence of bulky cargo, showing that they are due to a secretion-independent function of Tango1. Therefore, maintenance of ER/Golgi architecture and bulky cargo transport are the primary functions for Tango1.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rios-Barrera, L. D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sigurbjoernsdottir, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baer, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leptin, M. UNSPECIFIED orcid.org/0000-0001-7097-348X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210891
DOI:	10.1073/pnas.1711408114
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	114
Number:	48
Page Range:	S. E10389 - 10
Date:	2017
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX; COLLAGEN SECRETION; EXIT SITES; DROSOPHILA; CTAGE5; MORPHOGENESIS; MECHANISMS; TRANSPORT; DEFECTS; SPARC Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21089