Wu, Zhen, Liang, Shan, Song, Wen ORCID: 0000-0002-9498-2409, Lin, Guangzhong, Wang, Weiguang, Zhang, Heqiao ORCID: 0000-0003-0314-1573, Han, Zhifu and Chai, Jijie (2017). Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis. Front. Plant Sci., 8. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

Leucine-rich repeat receptor-like kinases (LRR-RLKs) are widespread in different plant species and play important roles in growth and development. Germination inhibition is vital for the completion of seed maturation and cell expansion is a fundamental cellular process driving plant growth. Here, we report genetic and structural characterizations of a functionally uncharacterized LRR-RLK, named GRACE (Germination Repression and Cell Expansion receptor-like kinase). Overexpression of GRACE in Arabidopsis exhibited delayed germination, enlarged cotyledons, rosette leaves and stubbier petioles. Conversely, these phenotypes were reversed in the T-DNA insertion knock-down mutant grace-1 plants. A crystal structure of the extracellular domain of GRACE (GRACE-LRR) determined at the resolution of 3.0 angstrom revealed that GRACE-LRR assumed a right-handed super-helical structure with an island domain (ID). Structural comparison showed that structure of the ID in GRACE-LRR is strikingly different from those observed in other LRR-RLKs. This structural observation implies that GRACE might perceive a new ligand for signaling. Collectively, our data support roles of GRACE in repressing seed germination and promoting cell expansion of Arabidopsis, presumably by perception of unknown ligand(s).

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wu, ZhenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liang, ShanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Song, WenUNSPECIFIEDorcid.org/0000-0002-9498-2409UNSPECIFIED
Lin, GuangzhongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, WeiguangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, HeqiaoUNSPECIFIEDorcid.org/0000-0003-0314-1573UNSPECIFIED
Han, ZhifuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-210995
DOI: 10.3389/fpls.2017.01999
Journal or Publication Title: Front. Plant Sci.
Volume: 8
Date: 2017
Publisher: FRONTIERS MEDIA SA
Place of Publication: LAUSANNE
ISSN: 1664-462X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
LEUCINE-RICH REPEAT; MEMBRANE H+-ATPASE; TRANSCRIPTION FACTORS; ABSCISIC-ACID; CRYSTAL-STRUCTURE; EXTRACELLULAR DOMAIN; NEGATIVE REGULATOR; STRESS TOLERANCE; PEPTIDE-HORMONE; PROTEIN-KINASEMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/21099

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