Universität zu Köln

Venter, Paul Christiaan, Nitsche, Frank, Domonell, Anne, Heger, Peter ORCID: 0000-0003-2583-2981 and Arndt, Hartmut ORCID: 0000-0003-2811-3595 (2017). The Protistan Microbiome of Grassland Soil: Diversity in the Mesoscale. Protist, 168 (5). S. 546 - 565. MUNICH: ELSEVIER GMBH. ISSN 1618-0941

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Abstract

Genomic data for less than one quarter of similar to 1.8 million named species on earth exist in public databases like GenBank. Little information exists on the estimated one million small sized (1-100 mu m) heterotrophic nanoflagellates and ciliates and their taxa-area relationship. We analyzed environmental DNA from 150 geo-referenced grassland plots representing topographical and land-use ranges typical for Central Europe. High through-put barcoding allowed the identification of operational taxonomic units (OTUs) at species level, with high pairwise identity to reference sequences (>= 99.7%), but also the identification of sequences at the genus (>= 97%) and class (>= 80%) taxonomic level. Species richness analyses revealed, on average, 100 genus level OTUs (332 unique individual read (UIR) and 56 class level OTUs per gram of soil sample in the mesoscale (1-1 000 km). Database shortfalls were highlighted by increased uncertain taxonomic lineages at lower resolution (>= 80% sequence identity). No single barcode occurred ubiquitously across all sites. Taxa-area relationships indicated that OTUs spread over the entire mesoscale were more similar than in the local scale and increased land-use (fertilization, mowing and grazing) promoted taxa-area separation. Only a small fraction of sequences strictly matched reference library sequences, suggesting a large protistan dark matter in soil which warrants further research. (C) 2017 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Venter, Paul Christiaan UNSPECIFIED UNSPECIFIED UNSPECIFIED Nitsche, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Domonell, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Heger, Peter UNSPECIFIED orcid.org/0000-0003-2583-2981 UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-213682
DOI:	10.1016/j.protis.2017.03.005
Journal or Publication Title:	Protist
Volume:	168
Number:	5
Page Range:	S. 546 - 565
Date:	2017
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1618-0941
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPERATIONAL TAXONOMIC UNITS; TAXA-AREA RELATIONSHIPS; HIGHLY DIVERSE; DNA; COMMUNITIES; SEQUENCES; CILIATE; BIOGEOGRAPHY; DISPERSAL; PARASITES Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21368