Rieckher, Matthias ORCID: 0000-0002-1945-9474 and Tavernarakis, Nektarios ORCID: 0000-0002-5253-1466 (2017). Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection. Bio-protocol, 7 (19). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325
Full text not available from this repository.Abstract
Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is delivered into the developing oocytes in the germline of adult hermaphrodites, which then generate transgenic animals among their offspring. In this protocol, we describe the microinjection procedure and the subsequent selection of transgenic progeny.
Item Type: | Journal Article | ||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-214528 | ||||||||||||
DOI: | 10.21769/BioProtoc.2565 | ||||||||||||
Journal or Publication Title: | Bio-protocol | ||||||||||||
Volume: | 7 | ||||||||||||
Number: | 19 | ||||||||||||
Date: | 2017 | ||||||||||||
Publisher: | BIO-PROTOCOL | ||||||||||||
Place of Publication: | SUNNYVALE | ||||||||||||
ISSN: | 2331-8325 | ||||||||||||
Language: | English | ||||||||||||
Faculty: | Unspecified | ||||||||||||
Divisions: | Unspecified | ||||||||||||
Subjects: | no entry | ||||||||||||
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Refereed: | Yes | ||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/21452 |
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