Universität zu Köln

Rieckher, Matthias ORCID: 0000-0002-1945-9474 and Tavernarakis, Nektarios ORCID: 0000-0002-5253-1466 (2017). Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection. Bio-protocol, 7 (19). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

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Abstract

Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is delivered into the developing oocytes in the germline of adult hermaphrodites, which then generate transgenic animals among their offspring. In this protocol, we describe the microinjection procedure and the subsequent selection of transgenic progeny.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rieckher, Matthias UNSPECIFIED orcid.org/0000-0002-1945-9474 UNSPECIFIED Tavernarakis, Nektarios UNSPECIFIED orcid.org/0000-0002-5253-1466 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-214528
DOI:	10.21769/BioProtoc.2565
Journal or Publication Title:	Bio-protocol
Volume:	7
Number:	19
Date:	2017
Publisher:	BIO-PROTOCOL
Place of Publication:	SUNNYVALE
ISSN:	2331-8325
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21452