Rieckher, Matthias ORCID: 0000-0002-1945-9474 and Tavernarakis, Nektarios ORCID: 0000-0002-5253-1466 (2017). Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection. Bio-protocol, 7 (19). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

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Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is delivered into the developing oocytes in the germline of adult hermaphrodites, which then generate transgenic animals among their offspring. In this protocol, we describe the microinjection procedure and the subsequent selection of transgenic progeny.

Item Type: Journal Article
CreatorsEmailORCIDORCID Put Code
Rieckher, MatthiasUNSPECIFIEDorcid.org/0000-0002-1945-9474UNSPECIFIED
Tavernarakis, NektariosUNSPECIFIEDorcid.org/0000-0002-5253-1466UNSPECIFIED
URN: urn:nbn:de:hbz:38-214528
DOI: 10.21769/BioProtoc.2565
Journal or Publication Title: Bio-protocol
Volume: 7
Number: 19
Date: 2017
Place of Publication: SUNNYVALE
ISSN: 2331-8325
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/21452


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