Universität zu Köln

Sanches, Pedro Gomes, op 't Veld, Roel C., de Graaf, Wolter, Strijkers, Gustav J. and Gruell, Holger (2017). Novel axolotl cardiac function analysis method using magnetic resonance imaging. PLoS One, 12 (8). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The salamander axolotl is capable of complete regeneration of amputated heart tissue. However, non-invasive imaging tools for assessing its cardiac function were so far not employed. In this study, cardiac magnetic resonance imaging is introduced as a non-invasive technique to image heart function of axolotls. Three axolotls were imaged with magnetic resonance imaging using a retrospectively gated Fast Low Angle Shot cine sequence. Within one scanning session the axolotl heart was imaged three times in all planes, consecutively. Heart rate, ejection fraction, stroke volume and cardiac output were calculated using three techniques: (1) combined long-axis, (2) short-axis series, and (3) ultrasound (control for heart rate only). All values are presented as mean +/- standard deviation. Heart rate (beats per minute) among different animals was 32.2+/-6.0 (long axis), 30.4+/-5.5 (short axis) and 32.7+/-4.9 (ultrasound) and statistically similar regardless of the imaging method (p > 0.05). Ejection fraction (%) was 59.6+/-10.8 (long axis) and 48.1+/-11.3 (short axis) and it differed significantly (p = 0.019). Stroke volume (mu l/beat) was 133.7+/-33.7 (long axis) and 93.2+/-31.2 (short axis), also differed significantly (p = 0.015). Calculations were consistent among the animals and over three repeated measurements. The heart rate varied depending on depth of anaesthesia. We described a new method for defining and imaging the anatomical planes of the axolotl heart and propose one of our techniques (long axis analysis) may prove useful in defining cardiac function in regenerating axolotl hearts.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sanches, Pedro Gomes UNSPECIFIED UNSPECIFIED UNSPECIFIED op 't Veld, Roel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED de Graaf, Wolter UNSPECIFIED UNSPECIFIED UNSPECIFIED Strijkers, Gustav J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruell, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221503
DOI:	10.1371/journal.pone.0183446
Journal or Publication Title:	PLoS One
Volume:	12
Number:	8
Date:	2017
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPINAL-CORD REGENERATION; HEART; SALAMANDER; SURVIVAL; BIOLOGY; DISEASE Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22150