Universität zu Köln

Peter, J., Kasper, C., Kaufholz, M., Buschow, R., Isensee, J., Hucho, T., Herberg, F. W., Schwede, F., Stein, C., Jordt, S. -E., Brackmann, M. and Spahn, V. (2017). Ankyrin-rich membrane spanning protein as a novel modulator of transient receptor potential vanilloid 1-function in nociceptive neurons. Eur. J. Pain, 21 (6). S. 1072 - 1087. HOBOKEN: WILEY. ISSN 1532-2149

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Abstract

BackgroundThe ion channel TRPV1 is mainly expressed in small diameter dorsal root ganglion (DRG) neurons, which are involved in the sensation of acute noxious thermal and chemical stimuli. Direct modifications of the channel by diverse signalling events have been intensively investigated, but little is known about the composition of modulating macromolecular TRPV1 signalling complexes. Here, we hypothesize that the novel adaptor protein ankyrin-rich membrane spanning protein/kinase D interacting substrate (ARMS) interacts with TRPV1 and modulates its function in rodent DRG neurons. MethodsWe used immunohistochemistry, electrophysiology, microfluorimetry and immunoprecipitation experiments to investigate TRPV1 and ARMS interactions in DRG neurons and transfected cells. ResultsWe found that TRPV1 and ARMS are co-expressed in a subpopulation of DRG neurons. ARMS sensitizes TRPV1 towards capsaicin in transfected HEK 293 cells and in mouse DRG neurons in a PKA-dependent manner. Using a combination of functional imaging and immunocytochemistry, we show that the magnitude of the capsaicin response in DRG neurons depends not only on TRPV1 expression, but on the co-expression of ARMS alongside TRPV1. ConclusionThese data indicate that ARMS is an important component of the signalling complex regulating the sensitivity of TRPV1. SignificanceThe study identifies ARMS as an important component of the signalling complex regulating the sensitivity of excitatory ion channels (TRPV1) in peripheral sensory neurons (DRG neurons) and transfected cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Peter, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kasper, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaufholz, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Buschow, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isensee, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hucho, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Herberg, F. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwede, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stein, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jordt, S. -E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brackmann, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Spahn, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-226974
DOI:	10.1002/ejp.1008
Journal or Publication Title:	Eur. J. Pain
Volume:	21
Number:	6
Page Range:	S. 1072 - 1087
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1532-2149
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED ION-CHANNEL; ROOT GANGLION NEURONS; CAPSAICIN RECEPTOR; SENSORY NEURONS; THERMAL HYPERALGESIA; INFLAMMATORY PAIN; TRPV1 CHANNEL; KINASE; PHOSPHORYLATION; KIDINS220/ARMS Multiple languages Anesthesiology; Clinical Neurology; Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22697