Universität zu Köln

Loebel, Madlen, Eckey, Maren, Sotzny, Franziska, Hahn, Elisabeth ORCID: 0000-0001-7168-1403, Bauer, Sandra, Grabowski, Patricia, Zerweck, Johannes, Holenya, Pavlo, Hanitsch, Leif G., Wittke, Kirsten, Borchmann, Peter, Rueffer, Jens-Ulrich, Hiepe, Falk, Ruprecht, Klemens, Behrends, Uta, Meindl, Carola, Volk, Hans-Dieter ORCID: 0000-0002-7743-6668, Reimer, Ulf and Scheibenbogen, Carmen (2017). Serological profiling of the EBV immune response in Chronic Fatigue Syndrome using a peptide microarray. PLoS One, 12 (6). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Background Epstein-Barr-Virus (EBV) plays an important role as trigger or cofactor for various autoimmune diseases. In a subset of patients with Chronic Fatigue Syndrome (CFS) disease starts with infectious mononucleosis as late primary EBV-infection, whereby altered levels of EBV-specific antibodies can be observed in another subset of patients. Methods We performed a comprehensive mapping of the IgG response against EBV comparing 50 healthy controls with 92 CFS patients using a microarray platform. Patients with multiple sclerosis (MS), systemic lupus erythematosus (SLE) and cancer-related fatigue served as controls. 3054 overlapping peptides were synthesised as 15-mers from 14 different EBV proteins. Array data was validated by ELISA for selected peptides. Prevalence of EBV sero-types was determined by qPCR from throat washing samples. Results EBV type 1 infections were found in patients and controls. EBV seroarray profiles between healthy controls and CFS were less divergent than that observed for MS or SLE. We found significantly enhanced IgG responses to several EBNA-6 peptides containing a repeat sequence in CFS patients compared to controls. EBNA-6 peptide IgG responses correlated well with EBNA-6 protein responses. The EBNA-6 repeat region showed sequence homologies to various human proteins. Conclusion Patients with CFS had a quite similar EBV IgG antibody response pattern as healthy controls. Enhanced IgG reactivity against an EBNA-6 repeat sequence and against EBNA-6 protein is found in CFS patients. Homologous sequences of various human proteins with this EBNA-6 repeat sequence might be potential targets for antigenic mimicry.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Loebel, Madlen UNSPECIFIED UNSPECIFIED UNSPECIFIED Eckey, Maren UNSPECIFIED UNSPECIFIED UNSPECIFIED Sotzny, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Hahn, Elisabeth UNSPECIFIED orcid.org/0000-0001-7168-1403 UNSPECIFIED Bauer, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Grabowski, Patricia UNSPECIFIED UNSPECIFIED UNSPECIFIED Zerweck, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Holenya, Pavlo UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanitsch, Leif G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wittke, Kirsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Borchmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueffer, Jens-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Hiepe, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruprecht, Klemens UNSPECIFIED UNSPECIFIED UNSPECIFIED Behrends, Uta UNSPECIFIED UNSPECIFIED UNSPECIFIED Meindl, Carola UNSPECIFIED UNSPECIFIED UNSPECIFIED Volk, Hans-Dieter UNSPECIFIED orcid.org/0000-0002-7743-6668 UNSPECIFIED Reimer, Ulf UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheibenbogen, Carmen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-227819
DOI:	10.1371/journal.pone.0179124
Journal or Publication Title:	PLoS One
Volume:	12
Number:	6
Date:	2017
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPSTEIN-BARR-VIRUS; SYSTEMIC-LUPUS-ERYTHEMATOSUS; CELLS IN-VIVO; EPITHELIAL-CELLS; ANTIBODY-RESPONSES; LYMPHOPROLIFERATIVE DISORDERS; NASOPHARYNGEAL CARCINOMA; RHEUMATOID-ARTHRITIS; MULTIPLE-SCLEROSIS; PERIPHERAL-BLOOD Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22781