Universität zu Köln

Lucas, Tina, Schaefer, Florian, Mueller, Patricia, Eming, Sabine A., Heckel, Alexander ORCID: 0000-0003-3541-4548 and Dimmeler, Stefanie ORCID: 0000-0002-1045-2436 (2017). Light-inducible antimiR-92a as a therapeutic strategy to promote skin repair in healing-impaired diabetic mice. Nat. Commun., 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

Full text not available from this repository.

Abstract

MicroRNAs (miRs) are small non-coding RNAs that post-transcriptionally control gene expression. Inhibition of miRs by antisense RNAs (antimiRs) might be a therapeutic option for many diseases, but systemic inhibition can have adverse effects. Here we show that light-activatable antimiRs efficiently and locally restricted target miR activity in vivo. We use an antimiR-92a and establish a therapeutic benefit in diabetic wound healing. AntimiR-92a is modified with photolabile protecting groups, so called 'cages'. Irradiation activates intradermally injected caged antimiR-92a without substantially affecting miR-92a expression in other organs. Light activation of caged antimiR-92a improves healing in diabetic mice to a similar extent as conventional antimiRs and derepresses the miR-92a targets Itga5 and Sirt1, thereby regulating wound cell proliferation and angiogenesis. These data show that light can be used to locally activate therapeutically active antimiRs in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lucas, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Patricia UNSPECIFIED UNSPECIFIED UNSPECIFIED Eming, Sabine A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heckel, Alexander UNSPECIFIED orcid.org/0000-0003-3541-4548 UNSPECIFIED Dimmeler, Stefanie UNSPECIFIED orcid.org/0000-0002-1045-2436 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-231410
DOI:	10.1038/ncomms15162
Journal or Publication Title:	Nat. Commun.
Volume:	8
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICRORNAS; DELIVERY; ANGIOGENESIS; ALPHA(5); GROWTH Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23141