Universität zu Köln

Snijder, Joost, Schuller, Jan M., Wiegard, Anika ORCID: 0000-0001-9674-0510, Lossl, Philip ORCID: 0000-0001-5062-7723, Schmelling, Nicolas ORCID: 0000-0002-6981-397X, Axmann, Ilka M., Plitzko, Juergen M., Foerster, Friedrich ORCID: 0000-0002-6044-2746 and Heck, Albert J. R. (2017). Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. Science, 355 (6330). S. 1181 - 1185. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Snijder, Joost UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuller, Jan M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiegard, Anika UNSPECIFIED orcid.org/0000-0001-9674-0510 UNSPECIFIED Lossl, Philip UNSPECIFIED orcid.org/0000-0001-5062-7723 UNSPECIFIED Schmelling, Nicolas UNSPECIFIED orcid.org/0000-0002-6981-397X UNSPECIFIED Axmann, Ilka M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plitzko, Juergen M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Foerster, Friedrich UNSPECIFIED orcid.org/0000-0002-6044-2746 UNSPECIFIED Heck, Albert J. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-236484
DOI:	10.1126/science.aag3218
Journal or Publication Title:	Science
Volume:	355
Number:	6330
Page Range:	S. 1181 - 1185
Date:	2017
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	1095-9203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CLOCK PROTEIN KAIB; CRYSTAL-STRUCTURE; PHOSPHORYLATION; DYNAMICS; COMPLEX; KAIABC; RHYTHM; EXPRESSION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23648