Universität zu Köln

Berninger, Markus T., Mohajerani, Pouyan, Kimm, Melanie, Masius, Stephan, Ma, Xiaopeng, Wildgruber, Moritz, Haller, Bernhard ORCID: 0000-0002-9723-393X, Anton, Martina, Imhoff, Andreas B., Ntziachristos, Vasilis, Henning, Tobias D. and Meier, Reinhard ORCID: 0000-0002-2169-4780 (2017). Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees. Eur. Radiol., 27 (3). S. 1105 - 1114. NEW YORK: SPRINGER. ISSN 1432-1084

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Abstract

To assess labelling efficiency of rabbit mesenchymal stem cells (MSCs) using the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) and detection of labelled MSCs for osteochondral defect repair in a rabbit model using fluorescence molecular tomography-X-ray computed tomography (FMT-XCT). MSCs were isolated from New Zealand White rabbits and labelled with DiR (1.25-20 mu g/mL). Viability and induction of apoptosis were assessed by XTT- and Caspase-3/-7-testing. Chondrogenic potential was evaluated by measurement of glycosaminoglycans. Labelled cells and unlabeled controls (n = 3) underwent FMT-XCT imaging before and after chondrogenic differentiation. Osteochondral defects were created surgically in rabbit knees (n = 6). Unlabeled and labelled MSCs were implanted in fibrin-clots and imaged by FMT-XCT. Statistical analyses were performed using multiple regression models. DiR-labelling of MSCs resulted in a dose-dependent fluorescence signal on planar images in trans-illumination mode. No significant reduction in viability or induction of apoptosis was detected at concentrations below 10 mu g DiR/mL (p > .05); the chondrogenic potential of MSCs was not affected (p > .05). FMT-XCT of labelled MSCs in osteochondral defects showed a significant signal of the transplant (p < .05) with additional high-resolution anatomical information about its osteochondral integration. FMT-XCT allows for detection of stem cell implantation within osteochondral regeneration processes. DiR-labelling of MSCs shows no toxic side effects or impairment of chondrogenesis. Fluorescence molecular tomography allows for detection of MSCs for osteochondral defect repair. FMT-XCT helps to improve evaluation of cell implantation and osteochondral regeneration processes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Berninger, Markus T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohajerani, Pouyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kimm, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Masius, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Ma, Xiaopeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Wildgruber, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Haller, Bernhard UNSPECIFIED orcid.org/0000-0002-9723-393X UNSPECIFIED Anton, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Imhoff, Andreas B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ntziachristos, Vasilis UNSPECIFIED UNSPECIFIED UNSPECIFIED Henning, Tobias D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Reinhard UNSPECIFIED orcid.org/0000-0002-2169-4780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-238633
DOI:	10.1007/s00330-016-4457-5
Journal or Publication Title:	Eur. Radiol.
Volume:	27
Number:	3
Page Range:	S. 1105 - 1114
Date:	2017
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1084
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RAY COMPUTED-TOMOGRAPHY; IN-VITRO; CARTILAGE DEFECTS; SEEDING DENSITY; MODEL; DIFFERENTIATION; PROLIFERATION; CHONDROCYTES; HYDROGELS; TRACKING Multiple languages Radiology, Nuclear Medicine & Medical Imaging Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23863