Lou, Ye, Rybniker, Jan, Sala, Claudia ORCID: 0000-0003-4031-4648 and Cole, Stewart T. (2017). EspC forms a filamentous structure in the cell envelope of Mycobacterium tuberculosis and impacts ESX-1 secretion. Mol. Microbiol., 103 (1). S. 26 - 39. HOBOKEN: WILEY-BLACKWELL. ISSN 1365-2958
Full text not available from this repository.Abstract
Pathogenicity of Mycobacterium tuberculosis (M. tb) is mediated by the ESX-1 secretion system, which exports EsxA and EsxB, the major virulence factors that are co-secreted with EspA and EspC. Functional information about ESX-1 components is scarce. Here, it was shown that EspC associates with EspA in the cytoplasm and membrane, then polymerizes during secretion from M. tb. EspC was localized by immuno-gold electron microscopy in whole cells or cryosections as a surface-exposed filamentous structure that seems to span the cell envelope. Consistent with these findings, purified EspC homodimerizes via disulphide bond formation, multimerizes and self-assembles into long filaments in vitro. The C-terminal domain is required for multimerization as truncation and selected point mutations therein impact EspC filament formation, thus reducing secretion of EsxA and causing attenuation of M. tb. The data are consistent with EspC serving either as a modulator of ESX-1 function or as a component of the secretion apparatus.
Item Type: | Journal Article | ||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-243788 | ||||||||||||||||||||
DOI: | 10.1111/mmi.13575 | ||||||||||||||||||||
Journal or Publication Title: | Mol. Microbiol. | ||||||||||||||||||||
Volume: | 103 | ||||||||||||||||||||
Number: | 1 | ||||||||||||||||||||
Page Range: | S. 26 - 39 | ||||||||||||||||||||
Date: | 2017 | ||||||||||||||||||||
Publisher: | WILEY-BLACKWELL | ||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||
ISSN: | 1365-2958 | ||||||||||||||||||||
Language: | English | ||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||
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Refereed: | Yes | ||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/24378 |
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