Brenzinger, Susanne, Dewenter, Lena, Delalez, Nicolas J., Leicht, Oliver, Berndt, Volker, Paulick, Anja, Berry, Richard M., Thanbichler, Martin, Armitage, Judith P., Maier, Berenike ORCID: 0000-0001-6971-9927 and Thormann, Kai M. (2016). Mutations targeting the plug-domain of the Shewanella oneidensis proton-driven stator allow swimming at increased viscosity and under anaerobic conditions. Mol. Microbiol., 102 (5). S. 925 - 939. HOBOKEN: WILEY-BLACKWELL. ISSN 1365-2958

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Abstract

Shewanella oneidensis MR-1 possesses two different stator units to drive flagellar rotation, the Na+-dependent PomAB stator and the H+-driven MotAB stator, the latter possibly acquired by lateral gene transfer. Although either stator can independently drive swimming through liquid, MotAB-driven motors cannot support efficient motility in structured environments or swimming under anaerobic conditions. Using pomAB cells we isolated spontaneous mutants able to move through soft agar. We show that a mutation that alters the structure of the plug domain in MotB affects motor functions and allows cells to swim through media of increased viscosity and under anaerobic conditions. The number and exchange rates of the mutant stator around the rotor were not significantly different from wild-type stators, suggesting that the number of stators engaged is not the cause of increased swimming efficiency. The swimming speeds of planktonic mutant MotAB-driven cells was reduced, and overexpression of some of these stators caused reduced growth rates, implying that mutant stators not engaged with the rotor allow some proton leakage. The results suggest that the mutations in the MotB plug domain alter the proton interactions with the stator ion channel in a way that both increases torque output and allows swimming at decreased pmf values.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Brenzinger, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dewenter, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Delalez, Nicolas J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leicht, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Berndt, VolkerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paulick, AnjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Berry, Richard M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thanbichler, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Armitage, Judith P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maier, BerenikeUNSPECIFIEDorcid.org/0000-0001-6971-9927UNSPECIFIED
Thormann, Kai M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-254963
DOI: 10.1111/mmi.13499
Journal or Publication Title: Mol. Microbiol.
Volume: 102
Number: 5
Page Range: S. 925 - 939
Date: 2016
Publisher: WILEY-BLACKWELL
Place of Publication: HOBOKEN
ISSN: 1365-2958
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BACTERIAL FLAGELLAR MOTOR; ESCHERICHIA-COLI; NA+-DRIVEN; VIBRIO-ALGINOLYTICUS; MOTIVE FORCE; PERIPLASMIC REGION; POLAR FLAGELLUM; BASAL BODY; PROTEIN; DYNAMICSMultiple languages
Biochemistry & Molecular Biology; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/25496

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