Universität zu Köln

Gomez, Juan Manuel, Chumakova, Lyubov, Bulgakova, Natalia A. and Brown, Nicholas H. (2016). Microtubule organization is determined by the shape of epithelial cells. Nat. Commun., 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Interphase microtubule organization is critical for cell function and tissue architecture. In general, physical mechanisms are sufficient to drive microtubule organization in single cells, whereas cells within tissues are thought to utilize signalling mechanisms. By improving the imaging and quantitation of microtubule alignment within developing Drosophila embryos, here we demonstrate that microtubule alignment underneath the apical surface of epithelial cells follows cell shape. During development, epidermal cell elongation and microtubule alignment occur simultaneously, but by perturbing cell shape, we discover that microtubule organization responds to cell shape, rather than the converse. A simple set of microtubule behaviour rules is sufficient for a computer model to mimic the observed responses to changes in cell surface geometry. Moreover, we show that microtubules colliding with cell boundaries zip-up or depolymerize in an angle-dependent manner, as predicted by the model. Finally, we show microtubule alignment responds to cell shape in diverse epithelia.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gomez, Juan Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Chumakova, Lyubov UNSPECIFIED UNSPECIFIED UNSPECIFIED Bulgakova, Natalia A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brown, Nicholas H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-258195
DOI:	10.1038/ncomms13172
Journal or Publication Title:	Nat. Commun.
Volume:	7
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language WING EPITHELIUM; PLANAR POLARITY; DROSOPHILA; AMNIOSEROSA; EFFICIENT; EPIDERMIS; DYNAMICS; GROWTH; ARRAYS; GENES Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25819