Koehler, Sybille, Brahler, Sebastian, Kuczkowski, Alexander, Binz, Julia, Hackl, Matthias J., Hagmann, Henning, Hoehne, Martin, Vogt, Merly C., Wunderlich, Claudia M., Wunderlich, F. Thomas, Schweda, Frank, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Brinkkoetter, Paul T. (2016). Single and Transient Ca2+ Peaks in Podocytes do not induce Changes in Glomerular Filtration and Perfusion. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

Full text not available from this repository.

Abstract

Chronic alterations in calcium (Ca2+) signalling in podocytes have been shown to cause proteinuria and progressive glomerular diseases. However, it is unclear whether short Ca2+ peaks influence glomerular biology and cause podocyte injury. Here we generated a DREADD (Designer Receptor Exclusively Activated by a Designer Drug) knock-in mouse line to manipulate intracellular Ca2+ levels. By mating to a podocyte-specific Cre driver we are able to investigate the impact of Ca2+ peaks on podocyte biology in living animals. Activation of the engineered G-protein coupled receptor with the synthetic compound clozapine-N-oxide (CNO) evoked a short and transient Ca2+ peak in podocytes immediately after CNO administration in vivo. Interestingly, this Ca2+ peak did neither affect glomerular perfusion nor filtration in the animals. Moreover, no obvious alterations in the glomerular morphology could be observed. Taken together, these in vivo findings suggest that chronic alterations and calcium overload rather than an induction of transient Ca2+ peaks contribute to podocyte disease.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Koehler, SybilleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brahler, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuczkowski, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Binz, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hackl, Matthias J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hagmann, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoehne, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vogt, Merly C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wunderlich, Claudia M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wunderlich, F. ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schweda, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schermer, BernhardUNSPECIFIEDorcid.org/0000-0002-5194-9000UNSPECIFIED
Benzing, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brinkkoetter, Paul T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-258293
DOI: 10.1038/srep35400
Journal or Publication Title: Sci Rep
Volume: 6
Date: 2016
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
FOCAL SEGMENTAL GLOMERULOSCLEROSIS; CONGENITAL NEPHROTIC SYNDROME; PROTEIN-COUPLED RECEPTORS; SLIT DIAPHRAGM; ACTIN CYTOSKELETON; CD2-ASSOCIATED PROTEIN; TRANSGENIC MICE; RENAL-FUNCTION; IN-VIVO; TRPC6Multiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/25829

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item