Universität zu Köln

Stevens, Holly, Williams, Ashley B. and Michael, W. Matthew (2016). Cell-Type Specific Responses to DNA Replication Stress in Early C. elegans Embryos. PLoS One, 11 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

To better understand how the cellular response to DNA replication stress is regulated during embryonic development, we and others have established the early C. elegans embryo as a model system to study this important problem. As is the case in most eukaryotic cell types, the replication stress response is controlled by the ATR kinase in early worm embryos. In this report we use RNAi to systematically characterize ATR pathway components for roles in promoting cell cycle delay during a replication stress response, and we find that these genetic requirements vary, depending on the source of stress. We also examine how individual cell types within the embryo respond to replication stress, and we find that the strength of the response, as defined by duration of cell cycle delay, varies dramatically within blastomeres of the early embryo. Our studies shed light on how the replication stress response is managed in the context of embryonic development and show that this pathway is subject to developmental regulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Stevens, Holly UNSPECIFIED UNSPECIFIED UNSPECIFIED Williams, Ashley B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Michael, W. Matthew UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-258415
DOI:	10.1371/journal.pone.0164601
Journal or Publication Title:	PLoS One
Volume:	11
Number:	10
Date:	2016
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CAENORHABDITIS-ELEGANS; DAMAGE RESPONSE; FORK PROTECTION; CHECKPOINT; DIVISION; ACTIVATION; TIMELESS; TIPIN; ATR Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25841