Universität zu Köln

Richter, Katharina Natalia, Rolke, Daniel, Blenau, Wolfgang ORCID: 0000-0002-6874-4730 and Baumann, Otto (2016). Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane. Cell Tissue Res., 366 (1). S. 163 - 175. NEW YORK: SPRINGER. ISSN 1432-0878

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Abstract

The honeybee hypopharyngeal gland consists in numerous units, each comprising a secretory cell and a canal cell. The secretory cell discharges its products into a convoluted tubular membrane system, the canaliculus, which is surrounded at regular intervals by rings of actin filaments. Using probes for various membrane components, we analyze the organization of the secretory cells relative to the apicobasal configuration of epithelial cells. The canaliculus was defined by labeling with an antibody against phosphorylated ezrin/radixin/moesin (pERM), a marker protein for the apical membrane domain of epithelial cells. Anti-phosphotyrosine visualizes the canalicular system, possibly by staining the microvillar tips. The open end of the canaliculus leads to a region in which the secretory cell is attached to the canal cell by adherens and septate junctions. The remaining plasma membrane stains for Na,K-ATPase and spectrin and represents the basolateral domain. We also used fluorophore-tagged phalloidin, anti-phosphotyrosine and anti-pERM as probes for the canaliculus in order to describe fine-structural changes in the organization of the canalicular system during the adult life cycle. These probes in conjunction with fluorescence microscopy allow the fast and detailed three-dimensional analysis of the canalicular membrane system and its structural changes in a developmental mode or in response to environmental factors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Richter, Katharina Natalia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rolke, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Blenau, Wolfgang UNSPECIFIED orcid.org/0000-0002-6874-4730 UNSPECIFIED Baumann, Otto UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-260735
DOI:	10.1007/s00441-016-2423-9
Journal or Publication Title:	Cell Tissue Res.
Volume:	366
Number:	1
Page Range:	S. 163 - 175
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0878
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEPTATE JUNCTION FUNCTION; APIS-MELLIFERA L.; SALIVARY-GLANDS; NA+/K+-ATPASE; ERM PROTEINS; COMPOUND EYE; PHOTORECEPTOR MORPHOGENESIS; ENDOPLASMIC-RETICULUM; ACTIN CYTOSKELETON; GLUCOSE-OXIDASE Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26073