Universität zu Köln

Schlereth, Simona L., Kremers, Stefan, Schroedl, Falk, Cursiefen, Claus and Heindl, Ludwig M. (2016). Characterization of Antigen-Presenting Macrophages and Dendritic Cells in the Healthy Human Sclera. Invest. Ophthalmol. Vis. Sci., 57 (11). S. 4878 - 4886. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. The sclera is mainly made of collagen and fibroblasts. The aim of this study was to analyze whether immune cells are present in the healthy human sclera. METHODS. Ten human anterior episcleral or stromal tissue samples from globe donors were immunohistochemically examined using confocal microscopy. The expression of the macrophage markers CD68, CD163 and CD11b, CD45 (a general leukocyte marker), MHCII (expressed by antigen-presenting cells [APCs]), CD11c (dendritic cell marker), lymphatic endothelium hyaluronan receptor-1 (LYVE1; expressed on lymphatic endothelium and macrophage subsets), chemokine receptor 7 (CCR7, a homing receptor for leukocytes), CXCL12 (expressed by activated leukocytes), CCR2 (a marker for inflammatory monocytes), and glial fibrillary acidic protein (GFAP; expressed by astrocytes) was analyzed and quantified. RESULTS. In the episclera, a high number of cells (>= 40 cells/mm(2)) were immunoreactive for CD68, CD45, MHCII, CCR7, LYVE1, and CD11b. Lower numbers (<20 cells/mm(2)) were positive for CXCL12, CCR2, and GFAP. The episclera showed a significantly higher number of cells compared to the stroma (P = 0.008). MHCII+ cells could be double positive for CCR7, CD45, CD11c, or CD11b and seldom CXCL12. Macrophages were most likely from the M1 type (CD68+, CD163 -). CONCLUSIONS. The healthy human sclera contains several macrophage populations, which can function as APCs, with the highest density being present in the episclera. Most cells express macrophage markers and may function as APCs. The presence of these cells might indicate that scleral immune cells are important for maintaining physiological functions in the eye and may potentially contribute to blood vessel homeostasis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schlereth, Simona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kremers, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroedl, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-263109
DOI:	10.1167/iovs.15-18552
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	57
Number:	11
Page Range:	S. 4878 - 4886
Date:	2016
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TUMOR-ASSOCIATED LYMPHANGIOGENESIS; CENTRAL-NERVOUS-SYSTEM; MARROW-DERIVED ELEMENTS; CILIARY-BODY-MELANOMAS; BONE-MARROW; PERIVASCULAR MACROPHAGES; MICROGLIAL CELLS; BLOOD-VESSELS; EXPRESSION; MONOCYTES Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26310