Universität zu Köln

Fatima, Azra, Xu, Guoxing, Nguemo, Filomain, Kuzmenkin, Alexey, Burkert, Karsten, Hescheler, Juergen and Saric, Tomo ORCID: 0000-0001-8344-1095 (2016). Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes. Stem Cell Res., 17 (2). S. 266 - 273. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1876-7753

Full text not available from this repository.

Abstract

We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyl-transferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of a-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) which can be generated at high purity with puromycin treatment and exhibit molecular and functional properties of immature heart muscle cells. This genetically modified iPSC line can be used for assessment of the utility of CMs for myocardial repair, pharmacological and toxicological applications and development of improved cardiac differentiation protocols. (C) 2016 The Authors. Published by Elsevier B.V.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fatima, Azra UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Guoxing UNSPECIFIED UNSPECIFIED UNSPECIFIED Nguemo, Filomain UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuzmenkin, Alexey UNSPECIFIED UNSPECIFIED UNSPECIFIED Burkert, Karsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-263125
DOI:	10.1016/j.scr.2016.07.007
Journal or Publication Title:	Stem Cell Res.
Volume:	17
Number:	2
Page Range:	S. 266 - 273
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1876-7753
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EMBRYONIC STEM-CELLS; SUSPENSION-CULTURE; DIFFERENTIATION; PURIFICATION; EXPRESSION; ENRICHMENT Multiple languages Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26312