Universität zu Köln

Horn, Thorsten and Panfilio, Kristen A. (2016). Novel functions for Dorsocross in epithelial morphogenesis in the beetle Tribolium castaneum. Development, 143 (16). S. 3002 - 3012. CAMBRIDGE: COMPANY OF BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

Epithelial morphogenesis, the progressive restructuring of tissue sheets, is fundamental to embryogenesis. In insects, not only embryonic tissues but also extraembryonic (EE) epithelia play a crucial role in shaping the embryo. In Drosophila, the T-box transcription factor Dorsocross (Doc) is essential for EE tissue maintenance and therefore embryo survival. However, Drosophila possesses a single amnioserosa, whereas most insects have a distinct amnion and serosa. How does this derived situation compare with Doc function in the ancestral context of two EE epithelia? Here, we investigate the Doc orthologue in the red flour beetle, Tribolium castaneum, which is an excellent model for EE tissue complement and for functional, fluorescent live imaging approaches. Surprisingly, we find that Tc-Doc controls all major events in Tribolium EE morphogenesis without affecting EE tissue specification or maintenance. These macroevolutionary changes in function between Tribolium and Drosophila are accompanied by regulatory network changes, where BMP signaling and possibly the transcription factor Hindsight are downstream mediators. We propose that the ancestral role of Doc was to control morphogenesis and discuss how Tc-Doc could provide spatial precision for remodeling the amnion-serosa border.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Horn, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Panfilio, Kristen A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-266532
DOI:	10.1242/dev.133280
Journal or Publication Title:	Development
Volume:	143
Number:	16
Page Range:	S. 3002 - 3012
Date:	2016
Publisher:	COMPANY OF BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SCUTTLE FLY MEGASELIA; GERM-BAND RETRACTION; DORSAL CLOSURE; DROSOPHILA-MELANOGASTER; CYCLORRHAPHAN FLIES; PCR DATA; GENES; AMNIOSEROSA; EMBRYOGENESIS; EXPRESSION Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26653