Universität zu Köln

Faden, Frederik ORCID: 0000-0002-8588-3142, Ramezani, Thomas ORCID: 0000-0003-4681-7844, Mielke, Stefan, Almudi, Isabel ORCID: 0000-0001-7978-4394, Nairz, Knud, Froehlich, Marceli S., Hoeckendorff, Joerg, Brandt, Wolfgang, Hoehenwarter, Wolfgang ORCID: 0000-0002-7669-7524, Dohmen, R. Juergen ORCID: 0000-0002-5756-6780, Schnittger, Arp ORCID: 0000-0001-7067-0091 and Dissmeyer, Nico (2016). Phenotypes on demand via switchable target protein degradation in multicellular organisms. Nat. Commun., 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversible and switch-like tuning of protein levels under physiological conditions in vivo. Thereby, developmental effects can be triggered and phenotypes on demand generated. The lt-degron was established to produce conditional and cell-type-specific phenotypes and is generally applicable in a wide range of organisms, from eukaryotic microorganisms to plants and poikilothermic animals. We have successfully applied this system to control the abundance and function of transcription factors and different enzymes by tunable protein accumulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Faden, Frederik UNSPECIFIED orcid.org/0000-0002-8588-3142 UNSPECIFIED Ramezani, Thomas UNSPECIFIED orcid.org/0000-0003-4681-7844 UNSPECIFIED Mielke, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Almudi, Isabel UNSPECIFIED orcid.org/0000-0001-7978-4394 UNSPECIFIED Nairz, Knud UNSPECIFIED UNSPECIFIED UNSPECIFIED Froehlich, Marceli S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoeckendorff, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Brandt, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehenwarter, Wolfgang UNSPECIFIED orcid.org/0000-0002-7669-7524 UNSPECIFIED Dohmen, R. Juergen UNSPECIFIED orcid.org/0000-0002-5756-6780 UNSPECIFIED Schnittger, Arp UNSPECIFIED orcid.org/0000-0001-7067-0091 UNSPECIFIED Dissmeyer, Nico UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-270535
DOI:	10.1038/ncomms12202
Journal or Publication Title:	Nat. Commun.
Volume:	7
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language END RULE PATHWAY; HEAT-INDUCIBLE DEGRON; ARABIDOPSIS-THALIANA; IN-VIVO; BETA-GLUCURONIDASE; HIGH-TEMPERATURE; DROSOPHILA-MELANOGASTER; CYTOSOLIC PROTEINS; DNA-REPLICATION; MESSENGER-RNA Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27053