Universität zu Köln

Goettig, Stephan, Riedel-Christ, Sara, Saleh, Ahmad, Kempf, Volkhard A. J. and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2016). Impact of bla(NDM-1) on fitness and pathogenicity of Escherichia coli and Klebsiella pneumoniae. Int. J. Antimicrob. Agents, 47 (6). S. 430 - 436. AMSTERDAM: ELSEVIER. ISSN 1872-7913

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Abstract

The objective of this study was to determine whether acquisition of New Delhi metallo-beta-lactamase-1 (NDM-1) has an impact on the fitness and virulence of Escherichia coli and Klebsiella pneumoniae. Growth kinetics and the cost of fitness of NDM-1 plasmid carriage were assessed in isogenic E. coli J53 and K. pneumoniae PRZ in vitro by pairwise competition assays. The pathogenicity of NDM-1-expressing E. coli and K. pneumoniae strains and their isogenic controls was analysed in vivo using a Galleria mellonella infection model. The cytotoxicity of NDM-1 was assessed in A549 human lung epithelial cells using the lactate dehydrogenase (LDH) assay. No differences in growth kinetics were recorded between NDM-1-expressing strains and controls (P = 0.92). A reduction in fitness of NDM-1-carrying strains was observed both for E. coli J53 and K. pneumoniae PRZ [selection rate constant (s) = -1.27 +/- 0.27 for E. coli J53 and -0.19 +/- 0.14 for K. pneumoniae PRZ; P < 0.0001]. Survival of G. mellonella larvae infected with NDM-1-expressing strains and controls was similar for E. coli J53 and K. pneumoniae PRZ. Cytotoxicity in A549 cells was not affected by NDM-1 expression (P > 0.05). The presence of bla(NDM-1) did not increase the virulence or cytotoxicity of isogenic strains. However, there was a considerable cost of fitness incurred by carriage of the pNDM-1 plasmid. Interestingly, the cost of fitness was significantly higher in E. coli J53 compared with K. pneumoniae PRZ. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Riedel-Christ, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Saleh, Ahmad UNSPECIFIED UNSPECIFIED UNSPECIFIED Kempf, Volkhard A. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-274554
DOI:	10.1016/j.ijantimicag.2016.02.019
Journal or Publication Title:	Int. J. Antimicrob. Agents
Volume:	47
Number:	6
Page Range:	S. 430 - 436
Date:	2016
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1872-7913
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language METALLO-BETA-LACTAMASE; COMBINATION; INFECTION; EFFICACY; ENTEROBACTERIACEAE; MODEL Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27455