Universität zu Köln

Gallas-Lindemann, Carmen ORCID: 0000-0002-6100-4342, Sotiriadou, Isaia, Plutzer, Judit ORCID: 0000-0003-3864-4197, Noack, Michael J., Mahmoudi, Mohammad Reza ORCID: 0000-0001-6820-1288 and Karanis, Panagiotis (2016). Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). Acta Trop., 158. S. 43 - 52. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1873-6254

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Abstract

Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n =185) and Cryptosporidium spp. (n=227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters. (C) 2016 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gallas-Lindemann, Carmen UNSPECIFIED orcid.org/0000-0002-6100-4342 UNSPECIFIED Sotiriadou, Isaia UNSPECIFIED UNSPECIFIED UNSPECIFIED Plutzer, Judit UNSPECIFIED orcid.org/0000-0003-3864-4197 UNSPECIFIED Noack, Michael J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahmoudi, Mohammad Reza UNSPECIFIED orcid.org/0000-0001-6820-1288 UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-275029
DOI:	10.1016/j.actatropica.2016.02.005
Journal or Publication Title:	Acta Trop.
Volume:	158
Page Range:	S. 43 - 52
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1873-6254
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PARVUM OOCYSTS; PROTOZOAN PARASITES; TOXOPLASMA-GONDII; DNA AMPLIFICATION; SAMPLES; SURFACE; DRINKING; GERMANY; IDENTIFICATION; TRANSMISSION Multiple languages Parasitology; Tropical Medicine Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27502