Universität zu Köln

Stahl, Elia, Bellwon, Patricia, Huber, Stefan, Schlaeppi, Klaus ORCID: 0000-0003-3620-0875, Bernsdorff, Friederike ORCID: 0000-0003-3457-2034, Vallat-Michel, Armelle, Mauch, Felix ORCID: 0000-0001-5150-2711 and Zeier, Juergen (2016). Regulatory and Functional Aspects of Indolic Metabolism in Plant Systemic Acquired Resistance. Mol. Plant., 9 (5). S. 662 - 682. CAMBRIDGE: CELL PRESS. ISSN 1752-9867

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Abstract

Tryptophan-derived, indolic metabolites possess diverse functions in Arabidopsis innate immunity to microbial pathogen infection. Here, we investigate the functional role and regulatory characteristics of indolic metabolism in Arabidopsis systemic acquired resistance (SAR) triggered by the bacterial pathogen Pseudomonas syringae. Indolic metabolism is broadly activated in both P. syringae-inoculated and distant, non-inoculated leaves. At inoculation sites, camalexin, indol-3-ylmethylamine (I3A), and indole-3-carboxylic acid (ICA) are the major accumulating compounds. Camalexin accumulation is positively affected by MYB122, and the cytochrome P450 genes CYP81F1 and CYP81F2. Local I3A production, by contrast, occurs via indole glucosinolate breakdown by PEN2-dependent and independent pathways. Moreover, exogenous application of the defense hormone salicylic acid stimulates I3A generation at the expense of its precursor indol-3-ylmethylglucosinolate (I3M), and the SAR regulator pipecolic acid primes plants for enhanced P. syringae-induced activation of distinct branches of indolic metabolism. In uninfected systemic tissue, the metabolic response is more specific and associated with enhanced levels of the indolics I3A, ICA, and indole-3-carbaldehyde (ICC). Systemic indole accumulation fully depends on functional CYP79B2/3, PEN2, and MYB34/51/122, and requires functional SAR signaling. Genetic analyses suggest that systemically elevated indoles are dispensable for SAR and associated systemic increases of salicylic acid. However, soil-grown but not hydroponically -cultivated cyp79b2/3 and pen2 plants, both defective in indolic secondary metabolism, exhibit pre-induced immunity, which abrogates their intrinsic ability to induce SAR.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Stahl, Elia UNSPECIFIED UNSPECIFIED UNSPECIFIED Bellwon, Patricia UNSPECIFIED UNSPECIFIED UNSPECIFIED Huber, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlaeppi, Klaus UNSPECIFIED orcid.org/0000-0003-3620-0875 UNSPECIFIED Bernsdorff, Friederike UNSPECIFIED orcid.org/0000-0003-3457-2034 UNSPECIFIED Vallat-Michel, Armelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Mauch, Felix UNSPECIFIED orcid.org/0000-0001-5150-2711 UNSPECIFIED Zeier, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276033
DOI:	10.1016/j.molp.2016.01.005
Journal or Publication Title:	Mol. Plant.
Volume:	9
Number:	5
Page Range:	S. 662 - 682
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1752-9867
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; SALICYLIC-ACID; DISEASE RESISTANCE; CAMALEXIN BIOSYNTHESIS; SECONDARY METABOLITES; GLUCOSINOLATE BIOSYNTHESIS; TRANSCRIPTION FACTOR; NONHOST RESISTANCE; PHYTOALEXIN ACCUMULATION; INDOLE-3-CARBOXYLIC ACID Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27603